{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have demonstrated that miR‑640 plays an important regulatory role in angiogenesis. In vascular endothelial cells, exposure to hydrogen sulfide (H₂S) downregulates miR‑640 expression, thereby relieving its inhibitory effect on hypoxia inducible factor 1‑α (HIF1A) and facilitating the VEGFR2–mTOR signaling cascade that promotes angiogenesis. Similarly, treatment with angiopoietin‑1 (Ang‑1) decreases miR‑640 levels, which in turn upregulates the expression of Zinc Finger Protein 91 (ZFP91) and enhances endothelial cell migration and capillary‑like tube formation. These findings suggest that miR‑640 functions as a negative regulator of proangiogenic signaling in response to different vascular stimuli."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn addition to its role in angiogenesis, miR‑640 has been implicated in tumor biology. In renal cell carcinoma, a circular RNA (circ_0054537) acts as a competing endogenous RNA to sponge miR‑640, leading to upregulation of neuronal pentraxin‑2 (NPTX2) and promoting malignant progression. Moreover, in glioma cells, reduced miR‑640 activity is associated with increased expression of slit guidance ligand 1 (SLIT1) and activation of the Wnt/β‑catenin signaling pathway, which in turn decreases cellular radiosensitivity. Collectively, these studies highlight miR‑640 as an important tumor suppressor whose downregulation can contribute to enhanced tumor growth and therapy resistance."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yu Zhou, Xing-Hui Li, Cai-Cai Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Hydrogen sulfide promotes angiogenesis by downregulating miR-640 via the VEGFR2/mTOR pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Am J Physiol Cell Physiol (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1152/ajpcell.00230.2015"}], "href": "https://doi.org/10.1152/ajpcell.00230.2015"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26879375"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26879375"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Sharon Harel, Veronica Sanchez-Gonzalez, Raquel Echavarria, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Roles of miR-640 and Zinc Finger Protein 91 (ZFP91) in Angiopoietin-1-Induced In Vitro Angiogenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cells (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3390/cells9071602"}], "href": "https://doi.org/10.3390/cells9071602"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32630670"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32630670"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Long Pei, Xianqiang Lv, Gaopei Jia, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Silencing circular RNA circ_0054537 and upregulating microRNA-640 suppress malignant progression of renal cell carcinoma via regulating neuronal pentraxin-2 (NPTX2)."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Bioengineered (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/21655979.2021.1984002"}], "href": "https://doi.org/10.1080/21655979.2021.1984002"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34565284"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34565284"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Yamei Zheng, Mingyue Xiao, Jingqiong Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Micro RNA-640 Targeting SLIT1 Enhances Glioma Radiosensitivity by Restraining the Activation of Wnt/β-Catenin Signaling Pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Br J Biomed Sci (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3389/bjbs.2022.10067"}], "href": "https://doi.org/10.3389/bjbs.2022.10067"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35996510"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35996510"}]}]}]}