| HGNC Family | Non-coding RNAs |
| Name | microRNA 648 |
| Description | microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009] |
| Summary |
{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nResearch has shown that miR-648 functions as a posttranscriptional regulator by targeting the 3′ untranslated region of endothelin-1 (ET-1) mRNA. In endothelial cells, elevated levels of placenta growth factor (PlGF)—a hallmark in sickle cell anemia patients with pulmonary hypertension—lead to a marked repression of miR-648. This downregulation of miR-648 results in an increased expression of the potent vasoconstrictor ET-1, thereby contributing to the development of pulmonary hypertension."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFurther investigation revealed that miR-648 is co‐transcribed with its host gene, MICAL3, from its distal promoter (P1), which is subject to positive regulation by the transcription factor PAX5. Downregulation of PAX5 activity by PlGF correlates with decreased miR-648 levels. Thus, the PlGF-induced reduction in PAX5-dependent transcription of miR-648 indirectly favors the stabilization and elevated levels of ET-1 mRNA, establishing a molecular link between microRNA regulation and vascular pathology in sickle cell anemia."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Chen Li, Caryn S Gonsalves, Marthe-Sandrine Eiymo Mwa Mpollo, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MicroRNA 648 Targets ET-1 mRNA and is cotranscriptionally regulated with MICAL3 by PAX5."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell Biol (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1128/MCB.01199-14"}], "href": "https://doi.org/10.1128/MCB.01199-14"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25403488"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25403488"}]}]}]}
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| Synonyms | MIRN648, HSA-MIR-648 |
| NCBI Gene ID | 693233 |
| API | |
| Download Associations | |
| Predicted Functions |
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| Co-expressed Genes |
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| Expression in Tissues and Cell Lines |
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MIR648 has 567 functional associations with biological entities spanning 4 categories (molecular profile, functional term, phrase or reference, cell line, cell type or tissue, gene, protein or microRNA) extracted from 11 datasets.
Click the + buttons to view associations for MIR648 from the datasets below.
If available, associations are ranked by standardized value
| Dataset | Summary | |
|---|---|---|
| CCLE Cell Line Gene CNV Profiles | cell lines with high or low copy number of MIR648 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset. | |
| COSMIC Cell Line Gene CNV Profiles | cell lines with high or low copy number of MIR648 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset. | |
| ENCODE Histone Modification Site Profiles | histone modification site profiles with high histone modification abundance at MIR648 gene from the ENCODE Histone Modification Site Profiles dataset. | |
| ENCODE Transcription Factor Binding Site Profiles | transcription factor binding site profiles with transcription factor binding evidence at the promoter of MIR648 gene from the ENCODE Transcription Factor Binding Site Profiles dataset. | |
| ENCODE Transcription Factor Targets | transcription factors binding the promoter of MIR648 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset. | |
| GeneSigDB Published Gene Signatures | PubMedIDs of publications reporting gene signatures containing MIR648 from the GeneSigDB Published Gene Signatures dataset. | |
| GTEx Tissue Gene Expression Profiles | tissues with high or low expression of MIR648 gene relative to other tissues from the GTEx Tissue Gene Expression Profiles dataset. | |
| JASPAR Predicted Transcription Factor Targets | transcription factors regulating expression of MIR648 gene predicted using known transcription factor binding site motifs from the JASPAR Predicted Transcription Factor Targets dataset. | |
| Klijn et al., Nat. Biotechnol., 2015 Cell Line Gene CNV Profiles | cell lines with high or low copy number of MIR648 gene relative to other cell lines from the Klijn et al., Nat. Biotechnol., 2015 Cell Line Gene CNV Profiles dataset. | |
| MotifMap Predicted Transcription Factor Targets | transcription factors regulating expression of MIR648 gene predicted using known transcription factor binding site motifs from the MotifMap Predicted Transcription Factor Targets dataset. | |
| Roadmap Epigenomics Histone Modification Site Profiles | histone modification site profiles with high histone modification abundance at MIR648 gene from the Roadmap Epigenomics Histone Modification Site Profiles dataset. | |
