{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nExcessive inflammation, as seen in periodontitis, impairs the osteogenic differentiation of human dental pulp stem cells (hDPSCs) through the upregulation of inflammatory cytokines such as TNF‑α. Under these inflammatory conditions, miR‑6512‑3p expression is increased, and this microRNA acts as a negative regulator of osteo/dentinogenic differentiation. In this context, the long noncoding RNA SNHG7, whose expression is downregulated by TNF‑α, normally functions as a molecular sponge that binds to miR‑6512‑3p, thereby reducing its abundance and mitigating its inhibitory effects on differentiation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFunctionally, the suppression of miR‑6512‑3p by SNHG7 under inflammatory conditions alleviates the TNF‑α‑induced impairment of hDPSC differentiation. This is evidenced by improvements in key osteogenic markers, including enhanced calcium deposition, increased alkaline phosphatase activity, and elevated expression of differentiation markers such as BSP, DSPP, DMP‑1, RUNX2, and OPN. Thus, miR‑6512‑3p plays an inhibitory role in hDPSC osteogenic differentiation in an inflammatory microenvironment, a process that can be counteracted by SNHG7, offering potential therapeutic targets for the treatment of periodontitis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Wenyu Chen "}, {"type": "b", "children": [{"type": "t", "text": "SNHG7 promotes the osteo/dentinogenic differentiation ability of human dental pulp stem cells by interacting with hsa-miR-6512-3p in an inflammatory microenvironment."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2021.09.081"}], "href": "https://doi.org/10.1016/j.bbrc.2021.09.081"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34653678"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34653678"}]}]}]}