{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent in silico and in vitro studies have revealed that miR‑6741‑3p plays a regulatory role in kidney disorders, particularly under the context of COVID‑19 complications. In one investigation, miR‑6741‑3p was found to target the 3′UTR of the APOL1 gene, where several single‐nucleotide polymorphisms (SNPs) were identified that can alter its binding efficiency. Furthermore, miR‑6741‑3p is predicted to regulate numerous APOL1‑related genes implicated in inflammatory, metabolic, and immune pathways, thereby affecting the pathogenesis of kidney conditions such as acute kidney injury, chronic kidney disease, collapsing glomerulopathy, and tubulointerstitial lesions associated with SARS‑CoV‑2 infection. These findings suggest that variations in miR‑6741‑3p and its target region may contribute to the increased kidney disease risk observed in certain populations under COVID‑19, highlighting its potential importance in renal pathology."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn a separate study focusing on oncogenesis, miR‑6741‑3p was identified as a tumor suppressor in oral squamous cell carcinoma (OSCC). Here, treatment with a DNA methyltransferase inhibitor reactivated its expression, which had been epigenetically silenced by hypermethylation. Functional analyses, including qRT‑PCR, Western blotting, and dual‑luciferase reporter assays, demonstrated that miR‑6741‑3p directly downregulates the oncogene SRSF3 at the translational level. In vitro and in vivo experiments further supported its role in inhibiting OSCC progression, suggesting that miR‑6741‑3p may serve as a promising biomarker and therapeutic target in OSCC as well as potentially in other malignancies."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Muhammad Safdar, Muhammad Sajjad Khan, Abdulkarim Yasin Karim, et al. "}, {"type": "b", "children": [{"type": "t", "text": "SNPs at 3'UTR of APOL1 and miR-6741-3p target sites associated with kidney diseases more susceptible to SARS-COV-2 infection: in silco and in vitro studies."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mamm Genome (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s00335-021-09880-6"}], "href": "https://doi.org/10.1007/s00335-021-09880-6"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34089082"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34089082"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Dhanashree Anil More, Nivedita Singh, Radha Mishra, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Intronic miR-6741-3p targets the oncogene SRSF3: Implications for oral squamous cell carcinoma pathogenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0296565"}], "href": "https://doi.org/10.1371/journal.pone.0296565"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "38781195"}], "href": "https://pubmed.ncbi.nlm.nih.gov/38781195"}]}]}]}