{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nAlthough the provided abstracts do not explicitly mention MIR6755, they describe the function of a coiled‐coil domain–containing protein—CCDC181—that appears to be vital for the proper differentiation of male germ cells. In murine models, CCDC181 was identified as a potential interacting partner of Hook1 and localizes to the microtubule-rich structures of elongating spermatids (i.e. the manchette) and the flagella of mature sperm. Its association with microtubules and interactions with catalytic subunits of protein phosphatase 1 suggest that it may help regulate cytoskeletal dynamics and possibly mediate aspects of ciliary motility during spermiogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFurther insights from knockout studies revealed that the absence of CCDC181 leads to severe defects in sperm development. Disruption of its function results in abnormal sperm head shaping, defective flagellum biogenesis, reduced sperm counts, and diminished motility, as well as multiple morphological abnormalities of the flagella. An interaction with the MMAF‐associated protein LRRC46, where CCDC181 regulates LRRC46 localization within the flagella, underscores its critical role in the biogenesis of the sperm tail and the maintenance of flagellar integrity."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Thomas Schwarz, Barbara Prieler, Johannes A Schmid, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Ccdc181 is a microtubule-binding protein that interacts with Hook1 in haploid male germ cells and localizes to the sperm tail and motile cilia."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Eur J Cell Biol (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.ejcb.2017.02.003"}], "href": "https://doi.org/10.1016/j.ejcb.2017.02.003"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28283191"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28283191"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Xiang-Jun Zhang, Xiao-Ning Hou, Jian-Teng Zhou, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CCDC181 is required for sperm flagellum biogenesis and male fertility in mice."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Zool Res (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.24272/j.issn.2095-8137.2024.075"}], "href": "https://doi.org/10.24272/j.issn.2095-8137.2024.075"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "39245650"}], "href": "https://pubmed.ncbi.nlm.nih.gov/39245650"}]}]}]}