{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nParathyroid hormone (PTH) has been shown to regulate osteoblast differentiation through a complex interplay between protein‐coding genes and noncoding RNAs, including both long noncoding RNAs (lncRNAs) and microRNAs (miRNAs). In human bone marrow stromal cells and osteoblastic MG63 cells, PTH treatment increases Runx2 mRNA levels—a key transcription factor driving osteoblast differentiation—and simultaneously upregulates the expression of lnc-SUPT3H-1:16. This lncRNA functions as a sponge for miR-6797-5p, a microRNA whose overexpression otherwise impairs osteoblast differentiation by targeting the 3′ untranslated region (3′UTR) of Runx2, effectively reducing Runx2 protein levels."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nBiochemical assays, including luciferase gene reporter experiments, confirmed that miR-6797-5p directly interacts with both lnc-SUPT3H-1:16 and Runx2 mRNA. Thus, by sequestering miR-6797-5p, the lncRNA relieves its inhibitory effect on Runx2, leading to sustained Runx2 expression and the subsequent promotion of osteoblast differentiation. Collectively, these findings illustrate how PTH orchestrates a finely tuned regulatory network involving lncRNA-mediated miRNA sponging to modulate the osteogenic program."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "B Arumugam, M Vishal, S Shreya, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Parathyroid hormone-stimulation of Runx2 during osteoblast differentiation via the regulation of lnc-SUPT3H-1:16 (RUNX2-AS1:32) and miR-6797-5p."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochimie (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.biochi.2018.12.006"}], "href": "https://doi.org/10.1016/j.biochi.2018.12.006"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30562548"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30562548"}]}]}]}