{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nmiR‑7704 has been identified as a nuclear microRNA enriched in the spliceosome fraction of breast cancer‐derived cells, where it acts as a tumor suppressor by negatively regulating the expression of cancer‑associated long noncoding RNAs such as HAGLR. In these cells, its inverse expression pattern with respect to HAGLR and the effects of its inhibition on HAGLR levels suggest that miR‑7704 impacts nuclear gene expression networks that modulate cell cycle progression and potentially other oncogenic processes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn addition to its nuclear role, host‑derived miR‑7704, when present in faeces, contributes to the progression of hepatic encephalopathy (HE) through microbiome modulation. Specifically, experiments in mice have shown that oral administration of hsa‑miR‑7704 exacerbates HE in a microbiome‐dependent manner by inhibiting the growth and adhesion of beneficial bacteria such as Bifidobacterium longum. This inhibition decreases the levels of acetate—a metabolite known to mitigate neuroinflammatory processes and ammonia production—thus promoting microglial activation and aggravating the HE phenotype."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Shelly Mahlab-Aviv, Keren Zohar, Yael Cohen, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Spliceosome-Associated microRNAs Signify Breast Cancer Cells and Portray Potential Novel Nuclear Targets."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Mol Sci (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3390/ijms21218132"}], "href": "https://doi.org/10.3390/ijms21218132"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33143250"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33143250"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Yuchong Wang, Yuyu Li, Longxian Lv, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "NPJ Biofilms Microbiomes (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41522-024-00487-8"}], "href": "https://doi.org/10.1038/s41522-024-00487-8"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "38396001"}], "href": "https://pubmed.ncbi.nlm.nih.gov/38396001"}]}]}]}