{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nIn a recent study, hsa‐miR-7973—an intronic microRNA expressed from the CYP19A1 gene—was shown to regulate target genes that are key modulators of the extracellular matrix and cell–cell interactions. Using a combination of transfection into the granulosa cell–derived KGN line, microarray expression profiling, in silico target prediction, and luciferase reporter assays, the investigators confirmed that hsa‐miR-7973 directly targets ADAM19, PXDN, and FMNL3, suggesting that this miRNA plays a role in shaping the microenvironment necessary for normal follicle development."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nThe expression pattern of hsa‐miR-7973 was further examined in human primary cumulus granulosa cell cultures, where its regulation following follicle stimulating hormone stimulation hints at a broader function in mediating the interactions between cells and their extracellular milieu during the dynamic process of ovarian follicle progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Ilmatar Rooda, Kati Hensen, Birgitta Kaselt, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Target prediction and validation of microRNAs expressed from FSHR and aromatase genes in human ovarian granulosa cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-020-59186-x"}], "href": "https://doi.org/10.1038/s41598-020-59186-x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32042028"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32042028"}]}]}]}