{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have revealed a novel role for hsa‐miR‐921 as a post‐transcriptional regulator. In one investigation, hsa‐miR‐921 was shown to interact with the 3′‐untranslated region of the carbonyl reductase 1 (CBR1) gene, leading to a significant reduction in luciferase reporter activity, protein expression, and enzymatic function in cells carrying the major rs9024 G allele. This miRNA’s regulatory effect on CBR1 was found to be dependent on the genotype, as a differential impact was observed when comparing cells with the G allele to those with the A allele."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nIn a separate study focusing on lung cancer, hsa‐miR‐921 was identified as a negative regulator of glutathione peroxidase 3 (GPx3) in A549 cells. Here, the miRNA bound directly to the GPx3 3′‐UTR, resulting in an inhibition of GPx3 expression—a key antioxidant enzyme involved in reducing oxidative stress—which may have implications for tumorigenesis and chemotherapeutic response."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n\nCollectively, these findings demonstrate that hsa‐miR‐921 contributes to the fine-tuning of gene expression by targeting the 3′‐UTRs of distinct genes involved in enzymatic metabolism and oxidative stress regulation. Its ability to modulate CBR1 expression in a genotype-dependent manner as well as to suppress antioxidant defense proteins like GPx3 suggests that hsa‐miR‐921 may play an important role in pharmacological drug metabolism and cancer pathophysiology."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "James L Kalabus, Qiuying Cheng, Javier G Blanco "}, {"type": "b", "children": [{"type": "t", "text": "MicroRNAs differentially regulate carbonyl reductase 1 (CBR1) gene expression dependent on the allele status of the common polymorphic variant rs9024."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0048622"}], "href": "https://doi.org/10.1371/journal.pone.0048622"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23133646"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23133646"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Jang-Yeol Choi, Byung Chull An, In Jung Jung, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MiR-921 directly downregulates GPx3 in A549 lung cancer cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Gene (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.gene.2019.02.086"}], "href": "https://doi.org/10.1016/j.gene.2019.02.086"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30898707"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30898707"}]}]}]}