{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nNDUFV1‐DT, originally annotated as c11orf72 (or FLJ90834), appears to be a recently emerged transcript specific to the human genome, with no orthologous sequence detected in closely related primate species. This transcript is situated adjacent to the housekeeping gene NDUFV1, yet its promoter displays a high level of methylation, in contrast to the unmethylated, constitutively active NDUFV1 promoter, suggesting a distinct regulatory regime."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nComprehensive expression analyses conducted across multiple normal (testicles, heart, brain, lung, bladder) and cancerous tissues (bladder tumor and testicular tumor) revealed no transcriptional activity for NDUFV1‐DT, raising doubts regarding its physiological role. Investigations into the protein product—via heterologous expression in human cell lines using both N‐ and C‐terminal TurboGFP fusion constructs—demonstrated that the encoded protein exhibits diffuse cellular distribution and lacks cytotoxic or promitotic activity."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nCollectively, these findings indicate that while NDUFV1‐DT may represent an example of a gene emerging through the gradual accumulation of point mutations during human evolution, its lack of detectable expression and functional activity in both normal and malignant contexts currently argue against a significant cellular role."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "K K Baskaev, R V Kholodenko, G V Malakhova, et al. "}, {"type": "b", "children": [{"type": "t", "text": "[Experimental analysis of human specific protein coding open reading frame c11orf72]."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Bioorg Khim (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1134/s1068162013020039"}], "href": "https://doi.org/10.1134/s1068162013020039"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23964515"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23964515"}]}]}]}