{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nThe long noncoding RNA NEXN‐AS1 has been shown to play a critical regulatory role in cardiovascular biology by modulating the expression of the actin‐binding protein NEXN. Reduced levels of both NEXN-AS1 and NEXN have been observed in human atherosclerotic plaques, and mechanistic studies indicate that NEXN-AS1 interacts with the chromatin remodeler BAZ1A at the NEXN promoter to upregulate its expression. Enhanced NEXN-AS1 levels lead to the inhibition of TLR4 oligomerization and NF-κB signaling, which in turn diminishes the expression of adhesion molecules and inflammatory cytokines as well as monocyte adhesion to endothelial cells. These findings are supported by both in vitro and in vivo experiments, including studies in ApoE-deficient mice where decreased NEXN levels were associated with aggravated atherosclerosis, suggesting that both NEXN-AS1 and its target gene NEXN may serve as promising therapeutic targets for atherosclerosis-related disorders."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "Disruptions in the structural integrity of NEXN-AS1 also appear to contribute to lung carcinogenesis. A large-scale meta-analysis of genome-wide association studies identified a novel risk single nucleotide polymorphism (rs114020893) located in the NEXN-AS1 region at 1p31.1 that is significantly associated with an increased risk for lung cancer. In silico analyses suggest that this SNP alters the secondary structure of NEXN-AS1, implicating it in the modulation of lung cancer susceptibility through mechanisms that remain to be fully elucidated."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yan-Wei Hu, Feng-Xia Guo, Yuan-Jun Xu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Clin Invest (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1172/JCI98230"}], "href": "https://doi.org/10.1172/JCI98230"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30589415"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30589415"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Hua Yuan, Hongliang Liu, Zhensheng Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A Novel Genetic Variant in Long Non-coding RNA Gene NEXN-AS1 is Associated with Risk of Lung Cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/srep34234"}], "href": "https://doi.org/10.1038/srep34234"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27713484"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27713484"}]}]}]}