{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nNeuroblastoma highly expressed long non‐coding RNA NHEG1 has been identified as significantly upregulated in neuroblastoma tissues compared to adjacent normal tissues. Its elevated expression correlates inversely with miR-665 levels and positively with HMGB1 expression, suggesting that NHEG1 plays a critical role in the aggressive behavior of neuroblastoma."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nMechanistically, NHEG1 functions as a competitive endogenous RNA (ceRNA) by sequestering miR-665. This sponging effect relieves the miR-665-mediated repression of HMGB1, subsequently promoting increased HMGB1 expression. As a result, NHEG1 indirectly enhances neuroblastoma cell proliferation, migration, and invasion."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFunctional assays in neuroblastoma cell lines further support the oncogenic role of NHEG1: its knockdown leads to increased miR-665 and decreased HMGB1 levels, accompanied by suppressed tumor cell aggressive phenotypes. Importantly, the inhibitory effects of NHEG1 knockdown can be rescued by the administration of a miR-665 inhibitor or by overexpressing HMGB1, underscoring the significance of the NHEG1/miR-665/HMGB1 regulatory axis in neuroblastoma progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yuqing Zhang, Yuping Hu, Aihong Pan, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Long non-coding RNA NHEG1/hsa-miR-665/HMGB1 axis is involved in the regulation of neuroblastoma progression."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Bioengineered (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/21655979.2021.1983277"}], "href": "https://doi.org/10.1080/21655979.2021.1983277"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34889712"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34889712"}]}]}]}