NSFL1C Gene

HGNC Family	UBX domain containing (UBXN)
Name	NSFL1 (p97) cofactor (p47)
Description	N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nNSFL1C encodes the p47 protein, a key adaptor of the AAA ATPase p97 that drives membrane fusion events critical for organelle integrity. p47 participates in Golgi reassembly by forming a tethering complex—together with p97 and partners such as FTCD—to facilitate the fusion of Golgi membranes during mitotic exit, thereby ensuring proper reassembly of the Golgi apparatus. This tethering function is central to its role in maintaining membrane organization and intracellular trafficking."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn addition to its role in membrane fusion, p47 functions as a negative regulator of inflammatory signaling. Upon cytokine stimulation (e.g., TNF‑α or IL‑1), p47 binds to Lys63-linked polyubiquitinated forms of NEMO, thereby targeting it for lysosomal degradation. This interaction limits IKK complex activation and curtails NF‑κB signaling, a mechanism that influences the magnitude of inflammatory responses and may be critical in conditions such as T-cell–driven pathology."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFurther expanding its functional repertoire, p47 is essential for the proper timing of centrosome maturation during the cell cycle. Studies have shown that depletion of p47 (and its orthologues) results in premature recruitment of Aurora A to centrosomes and misaligned mitotic spindles, indicating that p47 helps coordinate centrosome maturation with mitotic progression to safeguard correct cell division."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\np47 also contributes to the quality control of misfolded proteins by modulating endoplasmic reticulum-associated degradation (ERAD). As a member of the UBA‑UBX domain protein family, p47 displays substrate selectivity by accelerating the degradation of specific ERAD substrates, thereby influencing the cellular response to ER stress and maintaining proteostasis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nLastly, emerging evidence links p47 to neuroinflammatory processes. In microglia, p47 interacts with the translocator protein (TSPO), affecting microglial activation and polarization. This interplay is suggested to modulate inflammatory pathways relevant to neurodegenerative conditions such as Parkinson’s disease, thus broadening the impact of p47 from intracellular trafficking to the regulation of inflammation in the nervous system."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yayoi Kaneko, Kyohei Shimoda, Rafael Ayala, et al. "}, {"type": "b", "children": [{"type": "t", "text": "p97 and p47 function in membrane tethering in cooperation with FTCD during mitotic Golgi reassembly."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "EMBO J (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.15252/embj.2020105853"}], "href": "https://doi.org/10.15252/embj.2020105853"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33555040"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33555040"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Yuri Shibata, Masaaki Oyama, Hiroko Kozuka-Hata, et al. "}, {"type": "b", "children": [{"type": "t", "text": "p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncomms2068"}], "href": "https://doi.org/10.1038/ncomms2068"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22990857"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22990857"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Bidhan Sarkar, Ichiro Nishikata, Shingo Nakahata, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Degradation of p47 by autophagy contributes to CADM1 overexpression in ATLL cells through the activation of NF-κB."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-019-39424-7"}], "href": "https://doi.org/10.1038/s41598-019-39424-7"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30837480"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30837480"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Elsa Kress, Françoise Schwager, René Holtackers, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biol (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1083/jcb.201209107"}], "href": "https://doi.org/10.1083/jcb.201209107"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23649807"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23649807"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Eun Sil Park, Yung Joon Yoo, Muthukumar Elangovan "}, {"type": "b", "children": [{"type": "t", "text": "The opposite role of two UBA-UBX containing proteins, p47 and SAKS1 in the degradation of a single ERAD substrate, α-TCR."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell Biochem (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s11010-016-2860-5"}], "href": "https://doi.org/10.1007/s11010-016-2860-5"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27785701"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27785701"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Xue Xue, Rui Duan, Guoyan Zheng, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Translocator protein (18 kDa) regulates the microglial phenotype in Parkinson's disease through P47."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Bioengineered (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/21655979.2022.2068754"}], "href": "https://doi.org/10.1080/21655979.2022.2068754"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35475466"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35475466"}]}]}]}
Synonyms	DJ776F14.1, UBX1, UBXN2C
Proteins	NSF1C_HUMAN
NCBI Gene ID	55968
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

NSFL1C has 5,616 functional associations with biological entities spanning 9 categories (molecular profile, organism, functional term, phrase or reference, chemical, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA, sequence feature) extracted from 114 datasets.

Click the + buttons to view associations for NSFL1C from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

NSFL1C Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of NSFL1C gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of NSFL1C gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of NSFL1C gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of NSFL1C gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of NSFL1C gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of NSFL1C gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of NSFL1C gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of NSFL1C gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of NSFL1C gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with NSFL1C protein from the CCLE Cell Line Proteomics dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of NSFL1C gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of NSFL1C gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of NSFL1C gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing NSFL1C protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of NSFL1C gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing NSFL1C protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing NSFL1C protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing NSFL1C protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing NSFL1C protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with NSFL1C protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with NSFL1C protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of NSFL1C gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with NSFL1C gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with NSFL1C gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with NSFL1C gene/protein from the curated CTD Gene-Disease Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of NSFL1C protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by NSFL1C gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with NSFL1C gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with NSFL1C gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with NSFL1C gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with NSFL1C gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with NSFL1C gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at NSFL1C gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of NSFL1C gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of NSFL1C gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing NSFL1C from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of NSFL1C gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with NSFL1C gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing NSFL1C from the GeneSigDB Published Gene Signatures dataset.
	GEO Signatures of Differentially Expressed Genes for Diseases	disease perturbations changing expression of NSFL1C gene from the GEO Signatures of Differentially Expressed Genes for Diseases dataset.