{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nOSTM1-AS1 is a long noncoding RNA that is markedly upregulated in Wilms’ tumor and predominantly localized in the cytoplasm, suggesting a post‐transcriptional regulatory role in tumor biology. Its elevated expression, accompanied by increased levels of MELK and decreased miR-514a-3p, points to its involvement in critical oncogenic processes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nMechanistically, OSTM1-AS1 acts as a competing endogenous RNA by sponging miR-514a-3p, which relieves the microRNA-mediated suppression of MELK. This derepression of MELK enhances cellular proliferation and migration while simultaneously inhibiting apoptosis, thus contributing to tumor progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFunctionally, knockdown of OSTM1-AS1 in both in vitro assays and in vivo xenograft models resulted in reduced proliferation, diminished migratory capacity, increased apoptotic activity, and overall attenuation of tumor growth, underscoring the potential of the OSTM1-AS1/miR-514a-3p/MELK axis as a target for improved diagnosis and therapeutic intervention in Wilms’ tumor."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Jun Du, Jingjing Xiao, Yingquan Zhuo, et al. "}, {"type": "b", "children": [{"type": "t", "text": "LncRNA OSTM1-AS1 acts as an oncogenic factor in Wilms' tumor by regulating the miR-514a-3p/MELK axis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Anticancer Drugs (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1097/CAD.0000000000001320"}], "href": "https://doi.org/10.1097/CAD.0000000000001320"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35946509"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35946509"}]}]}]}