PHAF1 Gene

Name phagosome assembly factor 1
Description Predicted to be involved in Golgi to plasma membrane protein transport. Located in phagophore assembly site. [provided by Alliance of Genome Resources, Mar 2025]
Summary
{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nPHAF1—also referred to as MYTHO—is a highly conserved protein that plays a pivotal role in initiating macroautophagy, a key cellular recycling process that promotes stress resistance and healthy aging. Transcriptionally regulated and upregulated in the context of aging, PHAF1 functions as a scaffold that directly interacts with autophagy components such as WIPI2 and BCAS3. These interactions facilitate the assembly of the conjugation system at the nascent autophagosome (phagophore), ensuring that damaged or superfluous cellular components are efficiently degraded. Studies in Caenorhabditis elegans reveal that deletion of the PHAF1 homolog results in a dramatically shortened life span and decreased resilience to oxidative stress, underscoring its critical role in maintaining cellular homeostasis and organismal health."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Anais Franco-Romero, Valeria Morbidoni, Giulia Milan, et al. "}, {"type": "b", "children": [{"type": "t", "text": "C16ORF70/MYTHO promotes healthy aging in C.elegans and prevents cellular senescence in mammals."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Clin Invest (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1172/JCI165814"}], "href": "https://doi.org/10.1172/JCI165814"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "38869949"}], "href": "https://pubmed.ncbi.nlm.nih.gov/38869949"}]}]}]}
NCBI Gene ID 80262
API
Download Associations
Predicted Functions View PHAF1's ARCHS4 Predicted Functions.
Co-expressed Genes View PHAF1's ARCHS4 Predicted Functions.
Expression in Tissues and Cell Lines View PHAF1's ARCHS4 Predicted Functions.

Functional Associations

PHAF1 has 1,360 functional associations with biological entities spanning 4 categories (chemical, functional term, phrase or reference, cell line, cell type or tissue, gene, protein or microRNA) extracted from 16 datasets.

Click the + buttons to view associations for PHAF1 from the datasets below.

If available, associations are ranked by standardized value

Dataset Summary
Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles tissue samples with high or low expression of PHAF1 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
Carcinogenome Chemical Perturbation Carcinogenicity Signatures small molecule perturbations changing expression of PHAF1 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
CM4AI KOLF21J CRISPRi Gene Perturbation Atlas gene perturbations changing expression of PHAF1 gene from the CM4AI KOLF21J CRISPRi Gene Perturbation Atlas dataset.
CM4AI U2OS Cell Map Protein Localization Assemblies assemblies containing PHAF1 protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
GO Biological Process Annotations 2025 biological processes involving PHAF1 gene from the curated GO Biological Process Annotations2025 dataset.
GO Cellular Component Annotations 2025 cellular components containing PHAF1 protein from the curated GO Cellular Component Annotations 2025 dataset.
GTEx Tissue Gene Expression Profiles 2023 tissues with high or low expression of PHAF1 gene relative to other tissues from the GTEx Tissue Gene Expression Profiles 2023 dataset.
JASPAR Predicted Human Transcription Factor Targets 2025 transcription factors regulating expression of PHAF1 gene predicted using known transcription factor binding site motifs from the JASPAR Predicted Human Transcription Factor Targets dataset.
JASPAR Predicted Mouse Transcription Factor Targets 2025 transcription factors regulating expression of PHAF1 gene predicted using known transcription factor binding site motifs from the JASPAR Predicted Mouse Transcription Factor Targets 2025 dataset.
LINCS L1000 CMAP Chemical Perturbation Consensus Signatures small molecule perturbations changing expression of PHAF1 gene from the LINCS L1000 CMAP Chemical Perturbations Consensus Signatures dataset.
Replogle et al., Cell, 2022 K562 Essential Perturb-seq Gene Perturbation Signatures gene perturbations changing expression of PHAF1 gene from the Replogle et al., Cell, 2022 K562 Essential Perturb-seq Gene Perturbation Signatures dataset.
Replogle et al., Cell, 2022 K562 Genome-wide Perturb-seq Gene Perturbation Signatures gene perturbations changing expression of PHAF1 gene from the Replogle et al., Cell, 2022 K562 Genome-wide Perturb-seq Gene Perturbation Signatures dataset.
Rummagene Transcription Factor Associations 2026 transcription factors regulating expression of PHAF1 gene from the Rummagene Transcription Factor Associations 2026 dataset.
RummaGEO Drug Perturbation Signatures drug perturbations changing expression of PHAF1 gene from the RummaGEO Drug Perturbation Signatures dataset.
RummaGEO Gene Perturbation Signatures gene perturbations changing expression of PHAF1 gene from the RummaGEO Gene Perturbation Signatures dataset.
Sci-Plex Drug Perturbation Signatures drug perturbations changing expression of PHAF1 gene from the Sci-Plex Drug Perturbation Signatures dataset.