{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have highlighted that NEK9, a member of the NIMA‐related kinase family, plays a pivotal role as an adaptor protein facilitating autophagy‐dependent primary cilia formation. In one investigation, NEK9 was shown to interact with GABARAPs via its LC3-interacting region (LIR) and with the actin regulator MYH9. This interaction is crucial for the proper degradation of MYH9 during ciliogenesis, and mutations in the LIR impair the formation of primary cilia, a modification that appears to have evolved in land vertebrates."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nAnother study connected NEK9 with the replication stress response. Here, NEK9 was identified as a critical factor for maintaining genome integrity under replication stress conditions. Its depletion led to hypersensitivity to replication blocks, spontaneous DNA damage, and impaired CHK1 activation—underscoring NEK9’s importance in coordinating DNA repair and cell cycle checkpoint signaling in response to replication challenges."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n \nComplementary research further expanded the functional repertoire of NEK9 by demonstrating its central role in cell division. NEK9 is essential for proper spindle formation and chromosome alignment during both mitosis and meiosis. In mouse oocytes and early embryos, its depletion resulted in defective spindle organization, misaligned chromosomes, and cell cycle arrest, pointing to a function in microtubule nucleation at the spindle-organizing centers."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": " Additionally, NEK9, together with NEK2, contributes to endothelial barrier regulation; inhibition of these kinases alleviated lipopolysaccharide-induced hyperpermeability and myosin light chain 2 activation, highlighting a potential therapeutic target in severe lung injury."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yasuhiro Yamamoto, Haruka Chino, Satoshi Tsukamoto, et al. "}, {"type": "b", "children": [{"type": "t", "text": "NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41467-021-23599-7"}], "href": "https://doi.org/10.1038/s41467-021-23599-7"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34078910"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34078910"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Scott C Smith, Aleksandra V Petrova, Matthew Z Madden, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A gemcitabine sensitivity screen identifies a role for NEK9 in the replication stress response."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nucleic Acids Res (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/nar/gku840"}], "href": "https://doi.org/10.1093/nar/gku840"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25217585"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25217585"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Shang-Wu Yang, Chen Gao, Lei Chen, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Cycle (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.4161/cc.22690"}], "href": "https://doi.org/10.4161/cc.22690"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23159858"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23159858"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Nektarios Barabutis, Mohammad S Akhter "}, {"type": "b", "children": [{"type": "t", "text": "Involvement of NEK2 and NEK9 in LPS - induced endothelial barrier dysfunction."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Microvasc Res (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.mvr.2023.104651"}], "href": "https://doi.org/10.1016/j.mvr.2023.104651"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "38176677"}], "href": "https://pubmed.ncbi.nlm.nih.gov/38176677"}]}]}]}