{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nLong noncoding RNA PRKCQ‐AS1 is highly expressed in colorectal cancer tissues and cells, and its elevated levels are associated with a poor prognosis. Mechanistic studies have revealed that PRKCQ‐AS1 predominantly localizes to the cytoplasm, where it binds argonaute 2 and functions within the RNA-induced silencing complex. Through this action, PRKCQ‐AS1 acts as a competing endogenous RNA for miR‑1287‑5p, sequestering this microRNA and thereby reducing its tumor suppressor functions. This interaction results in enhanced proliferation and migratory capacities of colorectal cancer cells."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "In addition, miR‑1287‑5p normally targets the oncogene YBX1 for repression. By sponging miR‑1287‑5p, PRKCQ‑AS1 upregulates YBX1 at both the mRNA and protein levels, further promoting colorectal cancer progression. Rescue experiments conducted in vitro and in vivo have shown that overexpression of YBX1 partially reverses the inhibitory effects on proliferation and migration seen upon PRKCQ‑AS1 knockdown, underlining the critical role of the miR‑1287‑5p/YBX1 axis in mediating the oncogenic effects of PRKCQ‑AS1."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Guoce Cui, HongLi Zhao, Lina Li "}, {"type": "b", "children": [{"type": "t", "text": "Long noncoding RNA PRKCQ-AS1 promotes CRC cell proliferation and migration via modulating miR-1287-5p/YBX1 axis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biochem (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/jcb.29712"}], "href": "https://doi.org/10.1002/jcb.29712"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32619070"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32619070"}]}]}]}