{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRN7SL1, commonly known as 7SL RNA, plays a central role in protein targeting as an essential scaffold within the signal recognition particle (SRP). Structural studies have revealed that RN7SL1 provides the backbone for the hierarchical assembly of the SRP complex by binding to key proteins such as SRP19 and SRP54, which in turn facilitate proper RNA folding and the formation of functional motifs required for the co‐translational targeting of nascent secretory and membrane proteins."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn cancer, deregulation of RN7SL1 has been implicated in promoting aggressive tumor behavior. In the tumor microenvironment, activation of stromal signaling pathways can increase RN7SL1 expression and disrupt its normal association with protective RNA‐binding proteins, resulting in the accumulation of “unshielded” RN7SL1 in exosomes. This unshielded RNA acts as a damage-associated molecular pattern (DAMP) to stimulate innate immune receptors—such as RIG-I—thereby driving inflammatory responses that enhance tumor growth, metastasis, and resistance to therapy. In parallel, overexpression of RN7SL1 in tumor cells can repress p53 translation, a process that is normally counteracted by the transcriptional repressor FOXP3, establishing a regulatory loop with significant implications for tumor progression."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "3", "end_ref": "5"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nRN7SL1 is also co-opted by the innate antiviral machinery. In the context of retroviral infections, RN7SL1 is selectively packaged into viral particles where it serves as a critical cofactor for host cytidine deaminases such as APOBEC3G and APOBEC3F. This selective incorporation, mediated by interactions with the RNA-binding nucleocapsid domain of the viral Gag protein, is essential for the antiviral activity of these enzymes, as disruption of RN7SL1 packaging correlates with diminished antiviral defense."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFinally, emerging evidence highlights a role for RN7SL1 in neuroinflammatory processes. In Alzheimer’s disease models, knockdown of RN7SL1 has been shown to attenuate microglial activation in response to oligomeric amyloid‑β, leading to decreased production of proinflammatory cytokines and reduced neuronal damage. This positions RN7SL1 as a potential therapeutic target in the modulation of neuroimmune responses associated with neurodegeneration."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Chris Oubridge, Andreas Kuglstatter, Luca Jovine, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Crystal structure of SRP19 in complex with the S domain of SRP RNA and its implication for the assembly of the signal recognition particle."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/s1097-2765(02)00530-0"}], "href": "https://doi.org/10.1016/s1097-2765(02"}, {"type": "t", "text": "00530-0) PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12086622"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12086622"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Andreas Kuglstatter, Chris Oubridge, Kiyoshi Nagai "}, {"type": "b", "children": [{"type": "t", "text": "Induced structural changes of 7SL RNA during the assembly of human signal recognition particle."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Struct Biol (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/nsb843"}], "href": "https://doi.org/10.1038/nsb843"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12244299"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12244299"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Yanhui Yang, Jingli Cheng, Huizhu Ren, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Tumor FOXP3 represses the expression of long noncoding RNA 7SL."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2015.12.082"}], "href": "https://doi.org/10.1016/j.bbrc.2015.12.082"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26718402"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26718402"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Barzin Y Nabet, Yu Qiu, Jacob E Shabason, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cell.2017.06.031"}], "href": "https://doi.org/10.1016/j.cell.2017.06.031"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28709002"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28709002"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Jodie R Malcolm, Natasha K Leese, Philippa I Lamond-Warner, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Widespread association of ERα with RMRP and tRNA genes in MCF-7 cells and breast cancers."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Gene (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.gene.2022.146280"}], "href": "https://doi.org/10.1016/j.gene.2022.146280"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35143945"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35143945"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Tao Wang, Chunjuan Tian, Wenyan Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "7SL RNA mediates virion packaging of the antiviral cytidine deaminase APOBEC3G."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Virol (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1128/JVI.00892-07"}], "href": "https://doi.org/10.1128/JVI.00892-07"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17881443"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17881443"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Tao Wang, Chunjuan Tian, Wenyan Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Interaction with 7SL RNA but not with HIV-1 genomic RNA or P bodies is required for APOBEC3F virion packaging."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Mol Biol (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.jmb.2007.11.017"}], "href": "https://doi.org/10.1016/j.jmb.2007.11.017"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18067920"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18067920"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Hanyou Liu, Jingying Li, Xue Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Profiling of long non-coding RNAs in hippocampal-entorhinal system subfields: impact of RN7SL1 on neuroimmune response modulation in Alzheimer's disease."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Neuroinflammation (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s12974-024-03083-x"}], "href": "https://doi.org/10.1186/s12974-024-03083-x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "38582873"}], "href": "https://pubmed.ncbi.nlm.nih.gov/38582873"}]}]}]}