{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRNVU1-18 is an endoplasmic reticulum–resident protein that plays a central role in modulating lysosome dynamics. Experimental work in mouse brain extracts and embryonic fibroblasts demonstrates that RNVU1-18 regulates the formation of highly dynamic tubular lysosomes. It does so by promoting the degradation of specific lysosomal partners—most notably AP5Z1—thereby influencing the relative abundance of proteins such as spastizin at the lysosomal surface."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n\nRNVU1-18 further modulates lysosomal motility by indirectly coordinating the recruitment and balance of motor proteins. The degradation of AP5Z1 by RNVU1-18 adjusts the interactions of lysosomes with anterograde and retrograde motors, notably kinesin KIF13A and the dynein/dynactin complex component p150Glued. This delicate control mechanism ultimately determines the directionality of lysosomal trafficking."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n\nFinally, studies in polarized cortical neurons emphasize the physiological importance of RNVU1-18’s regulatory function. By ensuring the proper balance of lysosomal positioning and enhanced tubular lysosome formation, RNVU1-18 critically supports neuronal homeostasis and function."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Alexandre Pierga, Raphaël Matusiak, Margaux Cauhapé, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Spatacsin regulates directionality of lysosome trafficking by promoting the degradation of its partner AP5Z1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS Biol (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pbio.3002337"}], "href": "https://doi.org/10.1371/journal.pbio.3002337"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "37871017"}], "href": "https://pubmed.ncbi.nlm.nih.gov/37871017"}]}]}]}