{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nShroom2 functions as a scaffold protein that bridges the junctional membrane with the underlying actin cytoskeleton in epithelial cells. It directly binds to the MyTH4-FERM domain of myosin VIIa and F‐actin, and its association with the tight junction protein ZO-1 is critical for localizing and stabilizing cell-cell junctions. Notably, a specific region within Shroom2 protects actin filaments from disruption, emphasizing its role in preserving cytoskeletal integrity in sensory epithelia."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn endothelial cells, Shroom2 is equally essential for modulating cytoskeletal dynamics, contributing to the formation and maintenance of a contractile network at cell-cell adhesions. By directly engaging with Rho kinase (Rock), Shroom2 facilitates the recruitment of Rock and activated myosin II to adhesion sites, ensuring proper stress fiber organization and cellular tension. The loss of Shroom2 disrupts this balance, leading to diminished contractility and increased endothelial sprouting and migration, thereby enhancing angiogenic activity."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nCollectively, these studies underscore that Shroom2 plays a pivotal role in orchestrating cytoskeletal organization and junctional stability across multiple cell types. By acting as a molecular scaffold that couples adhesion molecules with contractile elements, Shroom2 is integral not only to the structural integrity of sensory epithelia but also to the dynamic regulation of vascular morphogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Raphaël Etournay, Ingrid Zwaenepoel, Isabelle Perfettini, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Sci (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/jcs.002568"}], "href": "https://doi.org/10.1242/jcs.002568"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17666436"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17666436"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Matthew J Farber, Ryan Rizaldy, Jeffrey D Hildebrand "}, {"type": "b", "children": [{"type": "t", "text": "Shroom2 regulates contractility to control endothelial morphogenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.E10-06-0505"}], "href": "https://doi.org/10.1091/mbc.E10-06-0505"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21248203"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21248203"}]}]}]}