{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent findings have uncovered a pivotal role for the long non‐coding RNA SLC25A5‑AS1 in gastric cancer, where its expression is markedly reduced in tumor tissues compared to adjacent normal tissues. This downregulation correlates with more aggressive clinicopathological features, such as increased tumor size, advanced TNM stage, and lymph node metastasis. Functionally, SLC25A5‑AS1 acts as a tumor suppressor by inhibiting cell proliferation, inducing G1 cell cycle arrest and apoptosis, and ultimately restraining tumor growth in vivo. Mechanistically, it serves as a competing endogenous RNA (ceRNA) that sequesters miR‑19a‑3p, thereby alleviating miR‑19a‑3p–mediated repression of PTEN and modulating the PI3K/AKT signaling pathway."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "Discerning a broader context of tissue regeneration and tumorigenesis, studies on the hematopoietic- and neurologic-expressed sequence 1 (Hn1) gene reveal its dynamic regulation in response to nerve injury. In models of facial nerve axotomy and vagus nerve injury, Hn1 mRNA is robustly upregulated in injured motoneurons, implicating this gene in the regenerative processes of the nervous system."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "Further investigations into Hn1 have demonstrated its multifaceted role in oncogenesis. In a murine melanoma model, siRNA-mediated depletion of Hn1 resulted in increased melanogenesis, perturbation of cell cycle regulators leading to G1/S arrest, and alterations in key signaling molecules such as c-Met, p27, p21, and p38 MAPK. Complementary studies in malignant gliomas confirmed Hn1 expression in high-grade tumors and showed that its depletion in glioma cells yielded significantly smaller tumor volumes in vivo, collectively suggesting that Hn1 functions as a suppressor of differentiation while promoting proliferative and regenerative cellular programs."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Xiwen Li, Xin Yan, Feng Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Down-regulated lncRNA SLC25A5-AS1 facilitates cell growth and inhibits apoptosis via miR-19a-3p/PTEN/PI3K/AKT signalling pathway in gastric cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Mol Med (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/jcmm.14200"}], "href": "https://doi.org/10.1111/jcmm.14200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30793479"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30793479"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Violetta Zujovic, Defang Luo, Henry V Baker, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The facial motor nucleus transcriptional program in response to peripheral nerve injury identifies Hn1 as a regeneration-associated gene."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Neurosci Res (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/jnr.20676"}], "href": "https://doi.org/10.1002/jnr.20676"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16267826"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16267826"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Katharine M Laughlin, Defang Luo, Che Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Hematopoietic- and neurologic-expressed sequence 1 expression in the murine GL261 and high-grade human gliomas."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Pathol Oncol Res (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s12253-008-9147-4"}], "href": "https://doi.org/10.1007/s12253-008-9147-4"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19145478"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19145478"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Katharine M Laughlin, Defang Luo, Che Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Hematopoietic- and neurologic-expressed sequence 1 (Hn1) depletion in B16.F10 melanoma cells promotes a differentiated phenotype that includes increased melanogenesis and cell cycle arrest."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Differentiation (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.diff.2009.04.001"}], "href": "https://doi.org/10.1016/j.diff.2009.04.001"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19427096"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19427096"}]}]}]}