{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nSNORD27 is a multifunctional C/D box small nucleolar RNA that, in its canonical role, guides the 2′-O-methylation of ribosomal RNA (specifically methylating A27 in 18S rRNA), thereby contributing to the proper modification and maturation of rRNA. However, recent work has revealed an additional, noncanonical function whereby a fraction of SNORD27 exists in a soluble nuclear complex lacking its typical binding partner fibrillarin. In this complex, SNORD27 directly interacts with pre-mRNA targets—most notably that of the transcription factor E2F7—regulating alternative splicing by competing with U1 small nuclear ribonucleoprotein (snRNP). Moreover, knockdown experiments indicate that suppression of SNORD27 leads to activation of previously silent exons in several other genes through extensive base complementarity across its entire sequence, suggesting that SNORD27 plays an expanded role in splicing regulation beyond its traditional rRNA processing duties."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn a clinical context, overexpression of SNORD27, along with several other SNORD family members, has been correlated with a shorter time to progression in smoldering myeloma. This association underscores the potential prognostic and possibly pathogenic significance of SNORD27 expression levels in hematologic malignancies, thereby linking its noncanonical regulatory roles to disease outcomes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Marina Falaleeva, Amadis Pages, Zaneta Matuszek, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.1519292113"}], "href": "https://doi.org/10.1073/pnas.1519292113"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26957605"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26957605"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Lucía López-Corral, María Victoria Mateos, Luis A Corchete, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Genomic analysis of high-risk smoldering multiple myeloma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Haematologica (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3324/haematol.2011.060780"}], "href": "https://doi.org/10.3324/haematol.2011.060780"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22331267"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22331267"}]}]}]}