{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nSPI1, which encodes the PU.1 transcription factor, is a master regulator of hematopoietic development. It is pivotal for the proper differentiation of myeloid lineages—including monocytes, macrophages, dendritic cells, osteoclasts, and cells of the erythroid and lymphoid compartments—and it functions by cooperatively interacting with other transcription factors. For example, PU.1 synergizes with NFATc1 to drive osteoclast‐specific gene expression, and it modulates the differentiation program through regulatory cascades involving microRNAs (e.g. miR‑424) and antisense RNAs. Moreover, subtle alterations or mutations in PU.1 disrupt normal myeloid maturation and have been linked to the pathogenesis of leukemias such as acute myeloid leukemia and acute promyelocytic leukemia."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "8"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn differentiated and mature immune cells, PU.1 continues to exert strong regulatory control. It regulates microglial gene expression and function, influencing neuroinflammatory pathways relevant to Alzheimer’s disease, while also guiding fibroblast activation that underpins fibrotic remodeling. In lymphoid compartments, PU.1 is involved in B‐cell antigen presentation and helps shape T‐cell subsets through its impact on microRNA networks such as miR‑155 and miR‑142‑3p. In addition, aberrant SPI1 fusion proteins have been identified in a subset of high‐risk pediatric T cell acute lymphoblastic leukemias, highlighting its importance in preventing malignant transformation."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "9", "end_ref": "18"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nThe activity and expression of PU.1 are tightly controlled by complex cis‐regulatory mechanisms and interactions with other transcriptional regulators. Distal enhancers, autoregulatory loops, and cooperative interactions with factors such as GATA‑1, C/EBPα, and various interferon regulatory factors (IRFs) all converge to fine‐tune PU.1 levels. Epigenetic modifications at the PU.1 promoter—such as dynamic histone acetylation and methylation—further modulate its expression during lineage commitment and cytokine production. In addition, PU.1 influences secondary gene networks by regulating noncoding RNAs, including members of the miR‑17‑92 cluster, miR‑22, and long noncoding RNAs, thereby cementing its role as a central hub in hematopoietic transcriptional regulation."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "19", "end_ref": "32"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nPU.1 also plays a crucial role in mediating responses to external stimuli and therapeutic interventions. In alveolar macrophages, its expression is regulated by GM‑CSF to maintain surfactant homeostasis, and targeting PU.1 can render leukemia cells more susceptible to apoptosis. Furthermore, a PU.1‐controlled regulatory axis involving miR‑142‑3p modulates autophagic pathways implicated in hepatocellular carcinoma drug resistance. In parallel, interactions of PU.1 with corepressors such as MeCP2 and oncogenes like EVI1 can repress its target gene transcription and contribute to aberrant myeloid differentiation in myelodysplastic syndromes."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "33", "end_ref": "37"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nEmerging evidence also underscores the broader immunomodulatory roles of PU.1. Variations in its activity have been associated with altered cytokine responses in autoimmune and neuropsychiatric conditions. For instance, abnormal PU.1 function has been linked to immune activation in schizophrenia and systemic lupus erythematosus, thereby influencing both innate and adaptive immune mechanisms through its control over antimicrobial peptide expression, cytokine networks, and the transcription of major histocompatibility complex regulators."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "38"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Venkateshwar A Reddy, Atsushi Iwama, Guergana Iotzova, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "Essential role of p38 mitogen-activated protein kinase in cathepsin K gene expression during osteoclastogenesis through association of NFATc1 and PU.1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M408795200"}], "href": "https://doi.org/10.1074/jbc.M408795200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15304486"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15304486"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Beatrice U Mueller, Thomas Pabst, José Fos, et al. "}, {"type": "b", "children": [{"type": "t", "text": "ATRA resolves the differentiation block in t(15;17) acute myeloid leukemia by restoring PU.1 expression."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Blood (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1182/blood-2005-07-3068"}], "href": "https://doi.org/10.1182/blood-2005-07-3068"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16352814"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16352814"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "A Rosa, M Ballarino, A Sorrentino, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.0706963104"}], "href": "https://doi.org/10.1073/pnas.0706963104"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18056638"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18056638"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Tania N Crotti, Sudarshana M Sharma, Joseph D Fleming, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "Loss of PU.1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Blood (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1182/blood.v99.8.3060"}], "href": "https://doi.org/10.1182/blood.v99.8.3060"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11929801"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11929801"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Ryan C Thompson, Melanie Herscovitch, Ian Zhao, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "A common haplotype lowers PU.1 expression in myeloid cells and delays onset of Alzheimer's disease."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Neurosci (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/nn.4587"}], "href": "https://doi.org/10.1038/nn.4587"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28628103"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28628103"}]}, {"type": "r", "ref": 15, "children": [{"type": "t", "text": "Masafumi Seki, Shunsuke Kimura, Tomoya Isobe, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "PU.1 regulates Alzheimer's disease-associated genes in primary human microglia."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Neurodegener (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s13024-018-0277-1"}], "href": "https://doi.org/10.1186/s13024-018-0277-1"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30124174"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30124174"}]}, {"type": "r", "ref": 17, "children": [{"type": "t", "text": "Thomas Wohlfahrt, Simon Rauber, Steffen Uebe, et al. "}, {"type": "b", "children": [{"type": "t", "text": "PU.1 controls fibroblast polarization and tissue fibrosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nature (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41586-019-0896-x"}], "href": "https://doi.org/10.1038/s41586-019-0896-x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30700907"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30700907"}]}, {"type": "r", "ref": 18, "children": [{"type": "t", "text": "Bilal Cakir, Yoshiaki Tanaka, Ferdi Ridvan Kiral, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41467-022-28043-y"}], "href": "https://doi.org/10.1038/s41467-022-28043-y"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35058453"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35058453"}]}, {"type": "r", "ref": 19, "children": [{"type": "t", "text": "Jian Du, Monika J Stankiewicz, Yang Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Novel combinatorial interactions of GATA-1, PU.1, and C/EBPepsilon isoforms regulate transcription of the gene encoding eosinophil granule major basic protein."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2002)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M204777200"}], "href": "https://doi.org/10.1074/jbc.M204777200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12202480"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12202480"}]}, {"type": "r", "ref": 20, "children": [{"type": "t", "text": "Nienke van der Stoep, Edwin Quinten, Marisa Marcondes Rezende, et al. "}, {"type": "b", "children": [{"type": "t", "text": "E47, IRF-4, and PU.1 synergize to induce B-cell-specific activation of the class II transactivator promoter III (CIITA-PIII)."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Blood (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1182/blood-2004-03-0790"}], "href": "https://doi.org/10.1182/blood-2004-03-0790"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15242870"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15242870"}]}, {"type": "r", "ref": 21, "children": [{"type": "t", "text": "Leonhard X Heinz, Barbara Platzer, Peter M Reisner, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Differential involvement of PU.1 and Id2 downstream of TGF-beta1 during Langerhans-cell commitment."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Blood (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1182/blood-2005-04-1721"}], "href": "https://doi.org/10.1182/blood-2005-04-1721"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16223775"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16223775"}]}, {"type": "r", "ref": 22, "children": [{"type": "t", "text": "Chu Wai Liew, Kasper D Rand, Raina J Y Simpson, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Molecular analysis of the interaction between the hematopoietic master transcription factors GATA-1 and PU.1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M602830200"}], "href": "https://doi.org/10.1074/jbc.M602830200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16861236"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16861236"}]}, {"type": "r", "ref": 23, "children": [{"type": "t", "text": "Christine Yeamans, Dehua Wang, Ido Paz-Priel, et al. "}, {"type": "b", "children": [{"type": "t", "text": "C/EBPalpha binds and activates the PU.1 distal enhancer to induce monocyte lineage commitment."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Blood (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1182/blood-2007-03-080291"}], "href": "https://doi.org/10.1182/blood-2007-03-080291"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17671233"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17671233"}]}, {"type": "r", "ref": 24, "children": [{"type": "t", "text": "Ulrich Steidl, Christian Steidl, Alexander Ebralidze, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A distal single nucleotide polymorphism alters long-range regulation of the PU.1 gene in acute myeloid leukemia."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Clin Invest (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1172/JCI30525"}], "href": "https://doi.org/10.1172/JCI30525"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17694175"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17694175"}]}, {"type": "r", "ref": 25, "children": [{"type": "t", "text": "Mathias Leddin, Chiara Perrod, Maarten Hoogenkamp, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "PU.1 and C/EBP(alpha) synergistically program distinct response to NF-kappaB activation through establishing monocyte specific enhancers."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.1017214108"}], "href": "https://doi.org/10.1073/pnas.1017214108"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21402921"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21402921"}]}, {"type": "r", "ref": 27, "children": [{"type": "t", "text": "Vit Pospisil, Karin Vargova, Juraj Kokavec, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "The ability of MLL to bind RUNX1 and methylate H3K4 at PU.1 regulatory regions is impaired by MDS/AML-associated RUNX1/AML1 mutations."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Blood (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1182/blood-2010-11-317909"}], "href": "https://doi.org/10.1182/blood-2010-11-317909"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22012064"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22012064"}]}, {"type": "r", "ref": 29, "children": [{"type": "t", "text": "Andreas Ramming, David Druzd, Jan Leipe, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Maturation-related histone modifications in the PU.1 promoter regulate Th9-cell development."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Blood (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1182/blood-2011-11-392589"}], "href": "https://doi.org/10.1182/blood-2011-11-392589"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22446486"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22446486"}]}, {"type": "r", "ref": 30, "children": [{"type": "t", "text": "Philipp B Staber, Pu Zhang, Min Ye, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Sustained PU.1 levels balance cell-cycle regulators to prevent exhaustion of adult hematopoietic stem cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.molcel.2013.01.007"}], "href": "https://doi.org/10.1016/j.molcel.2013.01.007"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23395001"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23395001"}]}, {"type": "r", "ref": 31, "children": [{"type": "t", "text": "Ming-Tai Chen, Hai-Shuang Lin, Chao Shen, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "The PU.1-Modulated MicroRNA-22 Is a Regulator of Monocyte/Macrophage Differentiation and Acute Myeloid Leukemia."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS Genet (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pgen.1006259"}], "href": "https://doi.org/10.1371/journal.pgen.1006259"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27617961"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27617961"}]}, {"type": "r", "ref": 33, "children": [{"type": "t", "text": "Tracey L Bonfield, Baisakhi Raychaudhuri, Anagha Malur, et al. "}, {"type": "b", "children": [{"type": "t", "text": "PU.1 regulation of human alveolar macrophage differentiation requires granulocyte-macrophage colony-stimulating factor."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Am J Physiol Lung Cell Mol Physiol (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1152/ajplung.00216.2003"}], "href": "https://doi.org/10.1152/ajplung.00216.2003"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12896880"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12896880"}]}, {"type": "r", "ref": 34, "children": [{"type": "t", "text": "Mitsuhiro Suzuki, Toshiyuki Yamada, Fumiko Kihara-Negishi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Direct association between PU.1 and MeCP2 that recruits mSin3A-HDAC complex for PU.1-mediated transcriptional repression."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncogene (2003)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/sj.onc.1207182"}], "href": "https://doi.org/10.1038/sj.onc.1207182"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "14647463"}], "href": "https://pubmed.ncbi.nlm.nih.gov/14647463"}]}, {"type": "r", "ref": 35, "children": [{"type": "t", "text": "Leopoldo Laricchia-Robbio, Kavitha Premanand, Ciro R Rinaldi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "EVI1 Impairs myelopoiesis by deregulation of PU.1 function."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Res (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1158/0008-5472.CAN-08-2562"}], "href": "https://doi.org/10.1158/0008-5472.CAN-08-2562"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19208846"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19208846"}]}, {"type": "r", "ref": 36, "children": [{"type": "t", "text": "Iléana Antony-Debré, Ananya Paul, Joana Leite, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "PU.1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Death Dis (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41419-018-0344-0"}], "href": "https://doi.org/10.1038/s41419-018-0344-0"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29472524"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29472524"}]}, {"type": "r", "ref": 38, "children": [{"type": "t", "text": "Pallavi Gupta, Gangenahalli U Gurudutta, Daman Saluja, et al. "}, {"type": "b", "children": [{"type": "t", "text": "PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Mol Med (2009)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/j.1582-4934.2009.00757.x"}], "href": "https://doi.org/10.1111/j.1582-4934.2009.00757.x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "19382896"}], "href": "https://pubmed.ncbi.nlm.nih.gov/19382896"}]}, {"type": "r", "ref": 39, "children": [{"type": "t", "text": "Karin Weigelt, Livia A Carvalho, Roos C Drexhage, et al. "}, {"type": "b", "children": [{"type": "t", "text": "TREM-1 and DAP12 expression in monocytes of patients with severe psychiatric disorders. EGR3, ATF3 and PU.1 as important transcription factors."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Brain Behav Immun (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbi.2011.03.006"}], "href": "https://doi.org/10.1016/j.bbi.2011.03.006"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21421043"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21421043"}]}]}]}