{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nSVIL-AS1 is a long noncoding RNA whose expression is markedly downregulated in lung adenocarcinoma tissues, a reduction that correlates with a more favorable prognosis for patients. Emerging evidence indicates that epitranscriptomic regulation, particularly via N6-methyladenosine (m6A) modifications, plays a critical role in lung cancer tumorigenesis. In lung adenocarcinoma cells, overexpression of SVIL-AS1 was shown to suppress cell proliferation and disrupt cell cycle progression, suggesting its function as a tumor suppressor."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nMechanistically, the m6A methyltransferase METTL3 enhances the stability of SVIL-AS1 transcripts through increased m6A modification. This stabilization leads to the downregulation of the transcription factor E2F1 and its downstream target genes by not only inhibiting their expression but also by accelerating E2F1 protein degradation. Notably, the anti-proliferative effects induced by SVIL-AS1 overexpression were reversible upon either E2F1 overexpression or METTL3 knockdown, thereby underscoring the interdependent regulatory axis among METTL3, SVIL-AS1, and E2F1 in lung cancer carcinogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Zedong Hu, Liang Zhu, Yilin Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Bioengineered (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/21655979.2022.2025697"}], "href": "https://doi.org/10.1080/21655979.2022.2025697"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35068325"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35068325"}]}]}]}