{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nTrafficking regulator of GLUT4-1 (TRARG1) is an important mediator within the insulin signaling network that contributes to the regulation of insulin-stimulated GLUT4 trafficking and insulin sensitivity. Biochemical and mass spectrometry analyses have revealed that TRARG1 undergoes dephosphorylation in response to insulin via a PI3K/Akt-dependent pathway, and it has been identified as a novel substrate for glycogen synthase kinase 3 (GSK3). In murine adipocytes, priming phosphorylation at serine 84 permits GSK3 to phosphorylate additional sites (serines 72, 76, and 80), a mechanism that is conserved in human TRARG1. Pharmacological inhibition of GSK3 increases the levels of cell surface GLUT4 under submaximal insulin stimulation, an effect that is significantly diminished when TRARG1 expression is reduced. Together, these findings underscore the pivotal role of TRARG1 in mediating key phosphorylation events that regulate GLUT4 trafficking in response to insulin."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Xiaowen Duan, Dougall M Norris, Sean J Humphrey, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem J (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1042/BCJ20220153"}], "href": "https://doi.org/10.1042/BCJ20220153"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35594055"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35594055"}]}]}]}