{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nIn triple‐negative breast cancer, WEE2‑AS1 is up‐regulated and functions as an oncogenic lncRNA by sponging miR‑32‑5p, thereby relieving the suppression of its downstream target TOB1. This axis promotes cell proliferation, inhibits apoptosis, and enhances migration and invasion, indicating its potential as a therapeutic target in this aggressive cancer subtype."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "In arteriosclerosis obliterans, WEE2‑AS1 is also significantly up‑regulated in both plasma and arterial tissues. In primary human vascular endothelial cells, its elevated expression modulates cell viability and cell cycle progression—knockdown enhances cell viability and shifts cell cycle distribution by altering key regulators such as phosphorylated cyclin-dependent kinase 1, WEE1, myelin transcription factor 1, and CDC25B—indicating an important role in endothelial function and vascular pathology."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "In colorectal cancer, cancer-associated fibroblasts secrete small extracellular vesicles enriched in WEE2‑AS1. Once internalized by tumor cells, exosomal WEE2‑AS1 acts as a modular scaffold that bridges the interaction between MOB1A and the E3 ubiquitin ligase praja2, promoting MOB1A degradation via the ubiquitin-proteasome pathway. This degradation dampens the Hippo pathway, resulting in nuclear accumulation of YAP and enhanced tumor cell proliferation and progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "In glioblastoma, WEE2‑AS1 is modified by m6A and its post‑transcriptional stabilization is mediated by the m6A reader IGF2BP3. The stabilized lncRNA in turn impedes CUL2-mediated ubiquitination of RPN2, leading to its protein stabilization and subsequent activation of the PI3K‑Akt signaling pathway. Importantly, targeting WEE2‑AS1 has been shown to improve the sensitivity of GBM cells to dasatinib, suggesting a promising combinatorial therapeutic strategy."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": "Emerging data in glioma further underscore the clinical relevance of WEE2‑AS1, where its expression has been associated with patient prognosis. Although the detailed mechanisms in glioma require further elucidation, the prognostic value of WEE2‑AS1 positions it as a potential biomarker for patient stratification and targeted interventions."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Rong Wang, Ziming Huang, Chongwei Qian, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "The N"}, {"type": "a", "children": [{"type": "t", "text": "sup"}], "href": "sup"}, {"type": "t", "text": "6"}, {"type": "a", "children": [{"type": "t", "text": "/sup"}], "href": "/sup"}, {"type": "t", "text": "-methyladenosine-mediated lncRNA WEE2-AS1 promotes glioblastoma progression by stabilizing RPN2."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Theranostics (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.7150/thno.74600"}], "href": "https://doi.org/10.7150/thno.74600"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36168628"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36168628"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Xuqiang Zhu, Di Chen, Yiyu Sun, et al. "}, {"type": "b", "children": [{"type": "t", "text": "LncRNA WEE2-AS1 is a diagnostic biomarker that predicts poor prognoses in patients with glioma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "BMC Cancer (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s12885-023-10594-y"}], "href": "https://doi.org/10.1186/s12885-023-10594-y"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36747161"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36747161"}]}]}]}