{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nHepatocellular carcinoma (HCC) remains a prevalent and challenging digestive system malignancy, and recent research has begun to investigate the potential roles of long non‐coding RNAs in its development. In one such study, ZNF385D‐AS2—a long non‐coding RNA previously predicted to function in various human diseases—was examined for its expression patterns and prognostic significance in liver cancer. This work represented the first exploration of ZNF385D‐AS2 in the context of HCC and set the stage for understanding its potential regulatory roles in tumor biology."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nThe investigation leveraged RNA‐Seq data from The Cancer Genome Atlas (TCGA) to reveal that ZNF385D‐AS2 is expressed at low levels in liver tumors. Low expression of this lncRNA was statistically associated with several clinical parameters—including sex, T stage, N stage, M stage, and overall clinical stage—and was linked to a significantly shorter median overall survival among patients. Furthermore, multivariate Cox analyses underscored its value as an independent prognostic indicator in HCC."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nBeyond its prognostic implications, predictive analyses using Gene Set Enrichment Analysis (GSEA), alongside co‐expression and competing endogenous RNA (ceRNA) network investigations, suggested that ZNF385D‐AS2 may regulate or be regulated by specific microRNAs, notably miR‑196 and miR‑182. This regulatory signaling axis could shed light on the molecular mechanisms underscoring liver tumorigenesis, reinforcing the potential of ZNF385D‐AS2 both as a prognostic biomarker and as an insight into disease pathogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Ze Zhang, Shouqian Wang, Yahui Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Low lncRNA ZNF385D‑AS2 expression and its prognostic significance in liver cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncol Rep (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3892/or.2019.7238"}], "href": "https://doi.org/10.3892/or.2019.7238"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31322274"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31322274"}]}]}]}