The process of coupling threonine to threonyl-tRNA in a mitochondrion, catalyzed by threonyl-tRNA synthetase. In tRNA aminoacylation, the amino acid is first activated by linkage to AMP and then transferred to either the 2'- or the 3'-hydroxyl group of the 3'-adenosine residue of the tRNA. (Gene Ontology, GO_0070159)
1 genes participating in the mitochondrial threonyl-tRNA aminoacylation biological process from the curated GO Biological Process Annotations 2025 dataset.
