Any process that decreases the frequency, rate or extent of myotube differentiation. Myotube differentiation is the process in which a relatively unspecialized cell acquires specialized features of a myotube cell. Myotubes are multinucleated cells that are formed when proliferating myoblasts exit the cell cycle, differentiate and fuse. (Gene Ontology, GO_0010832)
12 genes participating in the negative regulation of myotube differentiation biological process from the curated GO Biological Process Annotations 2025 dataset.
