Enrichment analysis of genes dysregulated in SGK1 KO
Canned Analysis
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General Information
AccessionDCA00024530
TitleEnrichment analysis of genes dysregulated in SGK1 KO
DescriptionAn enrichment analysis was performed on the top most dyresgulated genes determined by applying the principal angle method to compare gene expression between SGK1 KO cells and control cells in the Th17 -- Naïve Cd4+ Cd62lhigh Cd44low T cells were sorted using the BD FACSAria cell sorter. Sorted cells were activated with plate bound anti-Cd3 (2μg/ml) and anti-Cd28 (2μg/ml) in the presence of cytokines: 2ng/mL rhTGF-β1 (Miltenyi Biotec), 25ng/mL rmIl-6 (Miltenyi Biotec), 20ng/ml rmIl-23 (Miltenyi Biotec), and 20ng/ml rmIL-β1 (Miltenyi Biotec). After differentiation, the cells were then rested for 72 h and restimulated with IL-23 cell type.
Analysis URLhttp://amp.pharm.mssm.edu/PAEA?id=3092807
Upload DateSeptember 20, 2017
Uploadermaayanlab
Metadata
Analysis Curatorgszeto
CREEDS IDgene:1536
Cell TypeTh17 -- Naïve Cd4+ Cd62lhigh Cd44low T cells were sorted using the BD FACSAria cell sorter. Sorted cells were activated with plate bound anti-Cd3 (2μg/ml) and anti-Cd28 (2μg/ml) in the presence of cytokines: 2ng/mL
Gene Symbol (human)SGK1
Gene Symbol (mouse)Sgk1
Organismmouse
Perturbation TypeKO
Characteristic Direction parameters
Control SamplesGSM1075005, GSM1075006
Perturbed SamplesGSM1075007, GSM1075008
Tools used in the analysis
Dataset Accession
PAEA
Published November 2015
Enrichment analysis tool implementing the principal angle method
3879
Characteristic Direction
Published March 2014
A geometrical multivariate approach to identify differentially expressed genes
26829
Datasets used in the analysis
Keyword
Tool Name
GSE43956
Gene Expression Omnibus
Induction of pathogenic Th17 cells by salt inducible kinase SGK-1 (SGK-1 KO)
7
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