Enrichment analysis of genes upregulated in SGK1 KO
Canned Analysis
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General Information
AccessionDCA00064560
TitleEnrichment analysis of genes upregulated in SGK1 KO
DescriptionAn enrichment analysis was performed on the top 500 most upregulated genes identified by applying the Characteristic Direction method comparing gene expression between SGK1 KO cells and control cells in the Th17 -- Naïve Cd4+ Cd62lhigh Cd44low T cells were sorted using the BD FACSAria cell sorter. Sorted cells were activated with plate bound anti-Cd3 (2μg/ml) and anti-Cd28 (2μg/ml) in the presence of cytokines: 2ng/mL rhTGF-β1 (Miltenyi Biotec), 25ng/mL rmIl-6 (Miltenyi Biotec), 20ng/ml rmIl-23 (Miltenyi Biotec), and 20ng/ml rmIL-β1 (Miltenyi Biotec). After differentiation, the cells were then rested for 72 h and restimulated with IL-23 cell type.
Analysis URLhttp://amp.pharm.mssm.edu/Enrichr/enrich?dataset=1byqv
Upload DateSeptember 20, 2017
Uploadermaayanlab
Metadata
Analysis Curatorgszeto
CREEDS IDgene:1536
Cell TypeTh17 -- Naïve Cd4+ Cd62lhigh Cd44low T cells were sorted using the BD FACSAria cell sorter. Sorted cells were activated with plate bound anti-Cd3 (2μg/ml) and anti-Cd28 (2μg/ml) in the presence of cytokines: 2ng/mL
Gene Symbol (human)SGK1
Gene Symbol (mouse)Sgk1
Organismmouse
Perturbation TypeKO
Characteristic Direction parameters
Gene Setupregulated
Gene Set Size500
Control SamplesGSM1075005, GSM1075006
Perturbed SamplesGSM1075007, GSM1075008
Tools used in the analysis
Keyword
Dataset Accession
Enrichr
Published May 2016
A comprehensive gene set enrichment analysis web server 2016 update
7759
65
Characteristic Direction
Published March 2014
A geometrical multivariate approach to identify differentially expressed genes
26829
Datasets used in the analysis
Keyword
Tool Name
GSE43956
Gene Expression Omnibus
Induction of pathogenic Th17 cells by salt inducible kinase SGK-1 (SGK-1 KO)
7
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