Comparison of stem-cell derived retinal pigment epithelia (RPE) with human fetal retina pigment epithelium
Transcriptomics Dataset
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| Accession | GSE36695 |
| Title | Comparison of stem-cell derived retinal pigment epithelia (RPE) with human fetal retina pigment epithelium |
| Description | Retinal Pigment Epithelium (RPE) derived from two human embryonic stem cell lines, H1 and H9, were compared with human fetal RPE (hfRPE) using RNA-seq. Nominally, the transcriptome of H1-derived RPE showed greater overlap with hfRPE. For cells maintained in the medium used to differentiate RPE, 6.2%% (H1-RPE) and 4.2%% (H9-RPE) of the transcripts were expressed in amounts that were statistically different from hfRPE (false discovery rate: 5%%). After adaptation to the serum-free medium, SFM-1, only 1.0 %% (H1-RPE) and 1.9%% (H9-RPE) were expressed in amounts that were statistically different. For RPE signature genes, statistical differences were observed for 1.0 %% (H1-RPE) and 1.9%% (H9-RPE) of the transcripts. For some barrier-function related mRNAs the statistical differences were greater than these small differences would predict. For adhesion proteins and plasma membrane transporters, the differences were as great as 6.9%% and 4.3%%, respectively. After adaptation to SFM-1, the statistical differences between H1- and H9-RPE were only 0.4%% for all transcripts, 1.4%% for signature genes, and 0.7%% for membrane transporters. No statistical differences were observed for the transcripts related to tight junctions, adhesion junctions or ion channels. In summary, SFM-1 promoted the maturation of stem cell-derived RPE, and the statistical difference between two stem cell lines was minimal. |
| Landing Page | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE36695 |
| Repository | Gene Expression Omnibus |
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