GSE53244 | Datasets2Tools

General Information

Accession	GSE53244
Title	m6a peak analysis in normal, FTO deficient and five stages of adipogenesis (D-2/0/2/5/10) in Mouse embryo fibroblast 3T3-L1 pre-adipocytes
Description	Total RNA was extracted using TRI Reagent (Sigma). cDNA was synthesized by RevertAid First Strand cDNA Synthesis Kit with Oligo dT primers (K1622, Fermentas) following manufacturers recommendations. PCR reactions were carried out on a DNAEngine Thermal Cycler (PTC-0200G, Bio-Rad) in 25 l reaction volume containing 1 l cDNA, 200 nM primer pairs and components of TaKaRa Taq kit (R001A, Takara). All samples were analyzed in triplicate RT-qPCR.mRNAs were extracted using biotinylated poly(dT) oligo, followed by further removing of contaminated rRNA using RiboMinus Transcriptome Isolation Kit (K1550-02, Invitrogen). Then mRNAs were fragmented into 100-200nt length and subjected to immunoprecipitation with m6A specific antibody.The libraries were sequenced using HiSeq2000 (Illumina) in single-read mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane.
Landing Page	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53244
Repository	Gene Expression Omnibus