The chromatin modifier CHD8 targets autism risk genes during human neurodevelopment
Transcriptomics Dataset
FAIR Metrics
0 evaluations
General Information
| Accession | GSE57369 |
| Title | The chromatin modifier CHD8 targets autism risk genes during human neurodevelopment |
| Description | Whole-exome sequencing studies have implicated chromatin modifiers and transcriptional regulators in autism spectrum disorder (ASD) through the identification of de novo loss of function mutations in affected individuals. Many of these genes are co-expressed in mid-fetal human cortex, suggesting ASD risk genes converge in regulatory networks that are perturbed in ASD during neurodevelopment. To elucidate such networks we mapped promoters and enhancers bound by the chromodomain helicase CHD8, which is strongly enriched in ASD-associated de novo loss of function mutations, using ChIP-seq in mid-fetal human brain, human neural stem cells (hNSCs), and embryonic mouse cortex. We find that CHD8 targets are strongly enriched for ASD risk genes that converge in ASD-associated co-expression networks in human midfetal cortex. CHD8 knockdown in hNSCs results in significant dysregulation of ASD risk genes targeted by CHD8, as well as additional genes important for neurodevelopment, including members of the Wnt/-catenin signaling pathway. Integration of CHD8 binding data with genetic and gene co-expression data in ASD risk models provides support for additional ASD risk genes. Together, our results suggest that loss of CHD8 function contributes to ASD through regulatory perturbation of other ASD risk genes during human cortical development. |
| Landing Page | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57369 |
| Repository | Gene Expression Omnibus |
Related Datasets
GSE61476
FOXG1-dependent dysregulation of GABA/glutamate neuron differentiation in autism spectrum disorders
1
GSE61491
CHD8regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors [RNA-Seq]
1
GSE72442
Chd8 mediates cortical neurogenesis through transcriptional regulation of cell cycle and Wnt signaling genes
1
GSE28521
Transcriptomic Analysis of Autism Brain Reveals Convergent Molecular Pathology [array data]
21
Tools
used to analyze the dataset
Canned Analyses
generated with the dataset
FAIR Evaluation
Sorry, you must be signed in to evaluate this object.
