Opposing Roles of STAT1 and STAT3 in IL-21 Function in CD4+ T cells
Transcriptomics Dataset
FAIR Metrics
0 evaluations
General Information
| Accession | GSE63204 |
| Title | Opposing Roles of STAT1 and STAT3 in IL-21 Function in CD4+ T cells |
| Description | Interleukin-21 (IL-21) is a type 1 cytokine essential for immune cell differentiation and function. Although IL-21 can activate several STAT family transcription factors, previous studies focused mainly on the role of STAT3 in IL-21 signaling. Here, we investigated the role of STAT1 and show that STAT1 and STAT3 have at least partially opposing roles in IL-21 signaling in CD4+ T cells. IL-21 induced STAT1 phosphorylation, and this was augmented in Stat3-deficient CD4+ T cells. RNA-Seq analysis of CD4+ T cells from Stat1- and Stat3-deficient mice revealed that both STAT1 and STAT3 are critical for IL-21-mediated gene regulation. Expression of some genes, including Tbx21 and Ifng, was differentially regulated by STAT1 and STAT3, and interestingly, ChIP-Seq analysis showed that STAT3 binding at Tbx21 and Ifng loci was attenuated in Stat1-deficient cells. Moreover, opposing actions of STAT1 and STAT3 on IFN- expression in CD4+ T cells were demonstrated in vivo during chronic lymphocytic choriomeningitis (LCMV) infection. Finally, IL-21-mediated induction of STAT1 phosphorylation, as well as IFNG and TBX21 expression, were higher in CD4+ T cells from patients with autosomal dominant hyper-IgE syndrome (AD-HIES), which is caused by STAT3 deficiency. These data indicate an interplay between STAT1 and STAT3 in fine-tuning IL-21 actions. |
| Landing Page | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE63204 |
| Repository | Gene Expression Omnibus |
Related Datasets
GSE37646
Distinct STAT5 concentrations uniquely control establishment and differentiation of mammary epithelium during pregnancy
1
GSE65621
Asymmetry of STAT action in driving IL-27 and IL-6 transcriptional outputs and cytokine specificity
3
GSE76988
STAT5 is a key transcription factor IL-3-mediated inhibition of RANKL-induced osteoclastogenesis
1
GSE48970
Contribution of the STAT1alpha and STAT1beta isoforms to IFN-gamma mediated innate immunity
21
Tools
used to analyze the dataset
Canned Analyses
generated with the dataset
FAIR Evaluation
Sorry, you must be signed in to evaluate this object.
