Ambient O2 pressure induces NF-kB1/RelA related inflammatory response in human lung epithelial cells in vitro
Transcriptomics Dataset
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| Accession | GSE68378 |
| Title | Ambient O2 pressure induces NF-kB1/RelA related inflammatory response in human lung epithelial cells in vitro |
| Description | Purpose: Oxygen (O2) levels in cell culture conditions is typically 2-5 fold higher than the physiological O2 levels that most tissues experience in vivo. The ambient atmospheric O2 (21%%) is known to induce cell proliferation defects and cellular senescence in stem cell and primary cell cultures. Therefore, culturing these cells under lower O2 levels (2-9%%) is currently a standard practice. However, the non-cancerous immortalized cells and cancer cells, which evade cellular senescence are normally cultured under 21%% O2 levels and the effects of higher O2 levels on these cells are not fully understood. Methods: Gene expression (RNA seq transcriptomics) analysis of immortalized human bronchial epithelial (BEAS-2B) cells cultured at ambient 21%% O2 and lower 10%% O2 levels for 3 days and 3 weeks. Further the beneficial effects of cuturing cells under lower oxygen tension is evalulated Results: Our results show NF-B/RelA mediated activation of pro-inflammatory cytokines as a major outcome of cells being cultured 21%% O2. Moreover, we demonstrate increased RelA binding at the NF-B1/RelA target gene promoters at 21%% O2. Interestingly, contrary to cells cultutred at 21%% O2, external stress induced by H2O2 exposure did not induce inflammatory response in cells grown at 10%% O2, suggesting increased ability to handle external stress in cells cultured at lower O2 levels. |
| Landing Page | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68378 |
| Repository | Gene Expression Omnibus |
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