Intrinsic histone acetyltransferase activity of BRD4 is responsible for nucleosome eviction and transcriptional activation
Transcriptomics Dataset
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General Information
AccessionGSE71577
TitleIntrinsic histone acetyltransferase activity of BRD4 is responsible for nucleosome eviction and transcriptional activation
DescriptionPurpose: BRD4 is a chromatin reader and transcriptional activator of M/G1 genes.We discovered that BRD4 is a novel histone acetyltransferase that acetylates histones and evicts nucleosomes. Here, investigated genome-wide changes in nucleosome occupancy and transcription caused by BRD4 HAT activity . Method: Human U2OS cells were transfected with either wild type BRD4 (WT), a BRD4 HAT mutant (MT) or a empty vector (NT) in biological duplicates, harvested 18 hrs post transfection and subjected to global MNase-seq and RNA-seq analysis. Results: The results show that while wild type BRD4 reduces nucleosome occupancy and increases transcription genome-wide, the BRD4 HAT mutant does not. Conclusion: BRD4 HAT activity is responsible for evicting nucleosomes and activating transcription.
Landing Pagehttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71577
RepositoryGene Expression Omnibus
Tools used to analyze the dataset
Dataset Accession
ARCHS4
Published September 2017
ARCHS4 provides access to gene counts from HiSeq 2000 and HiSeq 2500 platforms for human and mouse experiments from GEO ...
4645
Clustergrammer
Published October 2017
Web-based tool for visualizing and analyzing high-dimensional data as interactive and shareable heatmaps
4645
Canned Analyses generated with the dataset
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Interactive heatmap visualization of RNA-seq dataset GSE71577
ARCHS4, Clustergrammer
Highly interactive web-based heatmap visualization of the top 500 most variable genes in t...
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