GSE74890 | Datasets2Tools

General Information

Accession	GSE74890
Title	Physical interaction between mutant calreticulin and the thrombopoietin receptor is required for transformation of hematopoietic cells
Description	Somatic mutations in calreticulin (CALR) are present in approximately 40%% of patients with myeloproliferative neoplasms (MPN). However, the mechanism by which mutant CALR is oncogenic is unknown. Here, we demonstrate that a megakaryocytic-specific MPN phenotype is induced when mutant CALR is over-expressed in mice and that the thrombopoietin receptor, MPL is required for mutant CALR driven transformation. Whole transcriptome analysis reveals enrichment of STAT signatures in mutant CALR transformed cells and JAK2 inhibitor treatment abrogates STAT activation. Employing extensive mutagenesis-based structure-function analysis we demonstrate that thepositively charged amino acids within the mutant CALR C-terminus are required for cellular transformation through facilitating physical interaction between mutant CALR and MPL.Together, our findings elucidate a novel mechanism of cancer pathogenesis.
Landing Page	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE74890
Repository	Gene Expression Omnibus