GSE74977 | Datasets2Tools

The LRF/ZBTB7A transcription factor is a BCL11A-independent repressor of fetal hemoglobin

Transcriptomics Dataset

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General Information

Accession	GSE74977
Title	The LRF/ZBTB7A transcription factor is a BCL11A-independent repressor of fetal hemoglobin
Description	Hemoglobinopathies, including sickle cell disease and _-thalassemia, are global public health concerns. Induction of fetal-type hemoglobin (HbF) is a promising means to treat these disorders; however, precisely how HbF expression is silenced in adult erythroid cells is not fully understood. Here, we show that the LRF/ZBTB7A transcription factor is a potent repressor of HbF production. LRF inactivation derepresses embryonic/fetal _-globin expression in mouse and human adult erythroid cells. We employed genome-wide analysis of the transcriptome, chromatin accessibility and LRF occupancy sites, and demonstrate that LRF occupies the _-globin loci and maintains nucleosome density necessary for _-globin silencing. LRF confers its repressive activity through a unique NuRD repressor complex independent of BCL11A. Strikingly, human erythroid lines lacking both LRF and BCL11A exhibited almost a complete switch in expression from adult- to fetal-type globin, suggesting that these two factors cumulatively represent the near entirety of _-globin repressive activity in adult erythroid cells.
Landing Page	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE74977
Repository	Gene Expression Omnibus

Tools used to analyze the dataset

Dataset Accession

ARCHS4

Published September 2017

ARCHS4 provides access to gene counts from HiSeq 2000 and HiSeq 2500 platforms for human and mouse experiments from GEO ...

4645

Clustergrammer

Published October 2017

Web-based tool for visualizing and analyzing high-dimensional data as interactive and shareable heatmaps

4645

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