Embryonic attenuated Wnt/-catenin signaling defines niche location and long-term stem cell fate in hair follicle
Transcriptomics Dataset
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General Information
AccessionGSE76223
TitleEmbryonic attenuated Wnt/-catenin signaling defines niche location and long-term stem cell fate in hair follicle
DescriptionLong-term adult stem cells sustain tissue homeostasis and regeneration throughout the lifetime of an organism. They are thought to originate from embryonic progenitors that acquired long-term self-renewal ability and multipotency at the end of organogenesis. We aim to mechanistically understand the embryonic origin of localized hair follicle stem cells. Here we discover embryonic progenitor cells occupying upper hair peg location; expressing a stem cell marker Nfatc1, contribute to long-term hair follicle stem cells, while the ones locating in the lower hair peg, expressing Shh, acquire differentiated cell fate. To understand the underlying mechanism leading to niche-induced HFSC specification, we profiled the expression of mRNA isolated from purified hair peg cells expressing Shh or Nfatc1. Based on DAVID functional gene annotation analysis, among genes that were expressed 2 fold more in Shh+ cells than in Nfatc1+ cells, we found that genes of the Wnt signaling pathway were enriched prominently. Base on these result and further genetic study, we suggest when hair placode develops to hair peg, a localized Wnt/-catenin signal free zone emerges. Embryonic progenitors residing in this region gain expression of stem cell markers compared to their precursors and become definitive long-term hair follicle stem cells.
Landing Pagehttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE76223
RepositoryGene Expression Omnibus
Tools used to analyze the dataset
Dataset Accession
ARCHS4
Published September 2017
ARCHS4 provides access to gene counts from HiSeq 2000 and HiSeq 2500 platforms for human and mouse experiments from GEO ...
4645
Clustergrammer
Published October 2017
Web-based tool for visualizing and analyzing high-dimensional data as interactive and shareable heatmaps
4645
Canned Analyses generated with the dataset
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Interactive heatmap visualization of RNA-seq dataset GSE76223
ARCHS4, Clustergrammer
Highly interactive web-based heatmap visualization of the top 500 most variable genes in t...
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