RNAseq Expression data for mouse liver, Phenobarbital exposed livers and resulting liver tumours.
Transcriptomics Dataset
FAIR Metrics
0 evaluations
General Information
| Accession | GSE77729 |
| Title | RNAseq Expression data for mouse liver, Phenobarbital exposed livers and resulting liver tumours. |
| Description | Through the analysis of mouse liver tumours promoted by distinct routes (DEN exposure alone, DEN exposure plus non-genotoxic insult with phenobarbital and non-alcoholic fatty liver disease); we report that the cancer associated hyper-methylated CGI events in mice are also predicated by silent promoters that are enriched for both the DNA modification 5-hydroxymethylcytosine (5hmC) and the histone modification H3K27me3 in normal liver. During cancer progression these CGIs undergo hypo-hydroxymethylation, prior to subsequent hyper-methylation; whilst retaining H3K27me3. A similar loss of promoter-core 5hmC is observed in Tet1 deficient mouse livers indicating that reduced Tet1 binding at CGIs may be responsible for the epigenetic dysregulation observed during hepatocarcinogenesis. Consistent with this reduced Tet1 protein levels are observed in mouse liver tumour lesions. As in human, DNA methylation changes at CGIs do not appear to be direct drivers of hepatocellular carcinoma progression in mice. Instead dynamic changes in H3K27me3 promoter deposition are strongly associated with tumour-specific activation and repression of transcription. Our data suggests that loss of promoter associated 5hmC in diverse liver tumours licences DNA methylation reprogramming at silent CGIs during cancer progression. |
| Landing Page | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE77729 |
| Repository | Gene Expression Omnibus |
Related Datasets
GSE49833
TET1 is a maintenance DNA demethylase that prevents methylation spreading in adult cells [RNA-seq]
1
GSE70089
Whole genome analysis of the methylome and hydroxymethylome in normal and malignant lung and liver [RNA-Seq]
1
GSE51901
Control of 5mC and 5hmC by TET dioxygenases in human embryonic carcinoma cells [Microarray]
21
Tools
used to analyze the dataset
Canned Analyses
generated with the dataset
FAIR Evaluation
Sorry, you must be signed in to evaluate this object.
