Nuclease footprints in sperm project past and future chromatin regulatory events
Transcriptomics Dataset
FAIR Metrics
0 evaluations
General Information
| Accession | GSE78075 |
| Title | Nuclease footprints in sperm project past and future chromatin regulatory events |
| Description | Nuclear remodeling to an extreme condensed state is a hallmark of spermatogenesis. This is achieved by varied degrees of replacement of histones with protamines. Regions retaining nucleosomes may be of functional significance. To determine potential roles for somatic-like chromatin in the paternal gamete, sperm from wild type and transgenic mice harboring a single copy insert of the human protamine cluster were subjected to Micrococcal Nuclease (MNase)-seq. Nuclease footprints linked robust endogenous protamine transcription and transgene suppression to its chromatin environment. Murine footprints were enriched within regulatory regions and sequences expressed in the early embryo. These were highlighted by Ctcf footprints that were enriched within chromatin domain boundaries and sites bound in testes and ESCs. In contrast, Ctcf footprints were absent in human and bull sperm. The continuity of Ctcf binding through the murine germline may permit rapid reconstitution of chromatin organization following fertilization. This likely reflects its preparation for early zygotic genome activation and comparatively accelerated preimplantation embryonic development program observed in mouse as compared to human. |
| Landing Page | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE78075 |
| Repository | Gene Expression Omnibus |
Related Datasets
GSE44267
Cohesin and CTCF Differentially Affect the Chromatin Architecture and Gene Expression in Human Cells
1
GSE55009
Paternal poly(ADP-ribose) metabolism modulates retention of inheritable sperm histones and early embryonic gene expression
1
GSE60095
Ribosome Profiling Reveals Pervasive Translation Outside of Annotated Protein-Coding Genes
2
GSE67892
CTCF and CohesinSA-1 Mark Active Promoters and Boundaries of Repressive Chromatin Domains in Primary Human Erythroid Cells [RNA-Seq]
1
GSE74537
Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals
1
Tools
used to analyze the dataset
Canned Analyses
generated with the dataset
FAIR Evaluation
Sorry, you must be signed in to evaluate this object.
