GSE87530 | Datasets2Tools

Transcriptome comparison of PAX6 ablated mouse beta cells to WT beta cells, ChIP-seq analysis of PAX6 bound sites both in mouse and human beta cell lines (Min6 and EndoC), and ChIP-seq analysis fo histone mark H3K9ac on mouse pancreatic beta cells.

Transcriptomics Dataset

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Accession	GSE87530
Title	Transcriptome comparison of PAX6 ablated mouse beta cells to WT beta cells, ChIP-seq analysis of PAX6 bound sites both in mouse and human beta cell lines (Min6 and EndoC), and ChIP-seq analysis fo histone mark H3K9ac on mouse pancreatic beta cells.
Description	Here, we report a key role for the transcription factor Pax6 in the maintenance of adult beta-cell identity and function. Pax6 is down regulated in beta-cells of diabetic db/db mice and in wild type mice treated with an insulin receptor antagonist, revealing metabolic control of expression. Deletion of Pax6 in beta-cells of adult mice leads to lethal hyperglycemia and ketosis, due to loss of beta-cell function and expansion of alpha-cells. Lineage tracing, transcriptome, and chromatin analyses show that Pax6 is a direct activator of beta-cell genes, maintaining mature beta-cell function and identity. In parallel, Pax6 binds promoters and enhancers to repress alternative islet cell genes including ghrelin, glucagon, and somatostatin. Chromatin analysis and shRNA-mediated gene suppression experiments indicate a similar function of PAX6 in human beta-cells. Reduced expression of Pax6 in metabolically stressed beta-cells may contribute to beta-cell failure and alpha-cell dysfunction in diabetes.
Landing Page	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE87530
Repository	Gene Expression Omnibus