General Information
| Description | A comprehensive design tool for CRISPR-mediated gene editing, repression and activation |
| Homepage | http://CRISPR-ERA.stanford.edu |
Publications
CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation
Summary:
The CRISPR/Cas9 system was recently developed as a powerful and flexible technology for targeted genome engineering, including genome editing (altering the genetic sequence) and gene regulation (without altering the genetic sequence). These applications require the design of single guide RNAs (sgRNAs) that are efficient and specific. However, this remains challenging, as it requires the consideration of many criteria. Several sgRNA design tools have been developed for gene editing, but currently there is no tool for the design of sgRNAs for gene regulation. With accumulating experimental data on the use of CRISPR/Cas9 for gene editing and regulation, we implement a comprehensive computational tool based on a set of sgRNA design rules summarized from these published reports. We report a genome-wide sgRNA design tool and provide an online website for predicting sgRNAs that are efficient and specific. We name the tool CRISPR-ERA, for clustered regularly interspaced short palindromic repeat-mediated editing, repression, and activation (ERA).
Availability and implementation
: http://CRISPR-ERA.stanford.edu.
Contact
: stanley.qi@stanford.edu or xwwang@tsinghua.edu.cn
Supplementary information:
Supplementary data are available at Bioinformatics online.
Related Tools
PrimerDesign-M
Published December 2014
A multiple-alignment based multiple-primer design tool for walking across variable genomes
SYNBADm
Published July 2016
A tool for optimization-based automated design of synthetic gene circuits
FAIR Evaluation
Sorry, you must be signed in to evaluate this object.
