General Information
| Description | A method for protein 3D structure reconstruction from contact maps, based on a non-Euclidean distance function |
| Homepage | http://iimcb.genesilico.pl/gdserver/GDFuzz3D/ |
Publications
GDFuzz3D: a method for protein 3D structure reconstruction from contact maps, based on a non-Euclidean distance function
Motivation:
To date, only a few distinct successful approaches have been introduced to reconstruct a protein 3D structure from a map of contacts between its amino acid residues (a 2D contact map). Current algorithms can infer structures from information-rich contact maps that contain a limited fraction of erroneous predictions. However, it is difficult to reconstruct 3D structures from predicted contact maps that usually contain a high fraction of false contacts.
Results:
We describe a new, multi-step protocol that predicts protein 3D structures from the predicted contact maps. The method is based on a novel distance function acting on a fuzzy residue proximity graph, which predicts a 2D distance map from a 2D predicted contact map. The application of a Multi-Dimensional Scaling algorithm transforms that predicted 2D distance map into a coarse 3D model, which is further refined by typical modeling programs into an all-atom representation. We tested our approach on contact maps predicted de novo by MULTICOM, the top contact map predictor according to CASP10. We show that our method outperforms FT-COMAR, the state-of-the-art method for 3D structure reconstruction from 2D maps. For all predicted 2D contact maps of relatively low sensitivity (60–84%), GDFuzz3D generates more accurate 3D models, with the average improvement of 4.87 Å in terms of RMSD.
Availability and implementation:
GDFuzz3D server and standalone version are freely available at http://iimcb.genesilico.pl/gdserver/GDFuzz3D/.
Contact:
iamb@genesilico.pl
Supplementary information:
Supplementary data are available at Bioinformatics online.
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