LongISLND
Computational Tool
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General Information
DescriptionIn silico sequencing of lengthy and noisy datatypes
Homepagehttp://bioinform.github.io/longislnd
Publications
LongISLND: in silico sequencing of lengthy and noisy datatypes
Bayo Lau; Marghoob Mohiyuddin; John C. Mu; Li Tai Fang; Narges Bani Asadi; Carolina Dallett; Hugo Y. K. Lam
Summary: LongISLND is a software package designed to simulate sequencing data according to the characteristics of third generation, single-molecule sequencing technologies. The general software architecture is easily extendable, as demonstrated by the emulation of Pacific Biosciences (PacBio) multi-pass sequencing with P5 and P6 chemistries, producing data in FASTQ, H5, and the latest PacBio BAM format. We demonstrate its utility by downstream processing with consensus building and variant calling.
Availability and Implementation: LongISLND is implemented in Java and available at http://bioinform.github.io/longislnd
Contact: hugo.lam@roche.com
Supplementary information: Supplementary data are available at Bioinformatics online.
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