PriSM
Computational Tool
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General Information
DescriptionA primer selection and matching tool for amplification and sequencing of viral genomes
Homepagehttp://www.broadinstitute.org/perl/seq/specialprojects/primerDesign.cgi
Publications
PriSM: a primer selection and matching tool for amplification and sequencing of viral genomes
Qing Yu; Elizabeth M. Ryan; Todd M. Allen; Bruce W. Birren; Matthew R. Henn; Niall J. Lennon
Summary: PriSM is a set of algorithms designed to select and match degenerate primer pairs for the amplification of viral genomes. The design of panels of hundreds of primer pairs takes just hours using this program, compared with days using a manual approach. PriSM allows for rapid in silico optimization of primers for downstream applications such as sequencing. As a validation, PriSM was used to create an amplification primer panel for human immunodeficiency virus (HIV) Clade B.
Availability: The program is freely available for use at: www.broadinstitute.org/perl/seq/specialprojects/primerDesign.cgi
Contact: nlennon@broadinstitute.org
Supplementary information: Supplementary data are available at Bioinformatics online.
DOI: https://doi.org/10.1093/bioinformatics/btq624
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