flowDensity
Computational Tool
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General Information
DescriptionReproducing manual gating of flow cytometry data by automated density-based cell population identification
Homepagehttp://master.bioconductor.org/packages/devel/bioc/html/flowDensity.html
Publications
flowDensity: reproducing manual gating of flow cytometry data by automated density-based cell population identification
Mehrnoush Malek; Mohammad Jafar Taghiyar; Lauren Chong; Greg Finak; Raphael Gottardo; Ryan R. Brinkman
Summary: flowDensity facilitates reproducible, high-throughput analysis of flow cytometry data by automating a predefined manual gating approach. The algorithm is based on a sequential bivariate gating approach that generates a set of predefined cell populations. It chooses the best cut-off for individual markers using characteristics of the density distribution. The Supplementary Data is linked to the online version of the manuscript.
Availability and implementation: R source code freely available through BioConductor (http://master.bioconductor.org/packages/devel/bioc/html/flowDensity.html.). Data available from FlowRepository.org (dataset FR-FCM-ZZBW).
Contact: rbrinkman@bccrc.ca
Supplementary information: Supplementary Data are available at Bioinformatics online.
Metrics:
6
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