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| Title / Description | Identification of transformation-related pathways in a breast epithelial cell model using a ribonomics approach. |
| Accession | GSE12215 |
| Summary | Employing MCT-1 oncogene mediated transformation of immortalized breast epithelial MCF10A cells; we characterized the largely reciprocal association of these two RBPs with target mRNAs and their influence on protein expression vis-à-vis cellular transformation. Using a ribonomics approach, we identified mRNAs from cancer-related pathways whose association with AUF1 and/or HuR were altered when comparing immortalized with transformed MCF10A cells. Significantly, we were able to demonstrate that knockdown of HuR expression using RNA interference, reduced anchorage-independent growth capacity in transformed MCF10A cells as well as decreased protein expression of a number of validated target genes. Our data demonstrate that the global alterations in binding of HuR and AUF1 with target transcripts have a critical role in post-transcriptional regulation of genes encoding proteins involved in breast epithelial cell transformation. |
| Organism | Homo sapiens |
| Platform | GPL6102 |
| Normalized | False |
| Parsed SOFT file |
| Differential expression method | Characteristic Direction |
| Cutoff | 500 |
| Organism | Homo sapiens |
| Cell | MCF10A |
| Cell subtype | MCF10A - pLXSN/HUR |
| Perturbation | pLXSN-MCT1/HUR |
| Gene | ELAVL1 (HUR) |
| Entrez gene | 1994 |
| Disease | Breast cancer |
| Principal Component Analysis | |
| Hierarchical clustering |
| Gene lists | Enrichr | L1000CDS2 | PAEA | |
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