ACAP2 Gene

HGNC Family	ArfGAPs, Pleckstrin homology domain containing (PLEKH), Ankyrin repeat domain containing (ANKRD)
Name	ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Description	Enables GTPase activator activity. Acts upstream of or within actin filament-based process. Located in ruffle. [provided by Alliance of Genome Resources, Mar 2025]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n ACAP2 is a multifunctional ADP‐ribosylation factor (Arf) GTPase‐activating protein that plays critical roles in regulating membrane trafficking and actin cytoskeleton dynamics. In its canonical function, ACAP2 preferentially inactivates Arf6 by stimulating GTP hydrolysis, thereby modulating processes such as PDGF‐induced dorsal ruffling and the formation of Arf6‐dependent protrusions at the cell periphery."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " In addition, during phagocytosis ACAP2 is recruited in a Rab35‐dependent manner to nascent phagocytic cups, where it orchestrates actin remodeling and membrane traffic by inactivating Arf6; these actions are essential for proper cup formation and efficient Fcγ receptor–mediated uptake of opsonized targets."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " Beyond its roles in endocytic recycling and phagosome formation, ACAP2 has also been implicated in host–pathogen interactions. For instance, studies in vaccinia virus have shown that a viral host‐range factor, K1L, interacts with the rabbit homolog of ACAP2, linking ACAP2 to viral replication processes and underscoring its relevance in host defense mechanisms."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Moreover, a circular RNA derived from the ACAP2 gene (circ ACAP2) is upregulated in cardiomyocytes following myocardial infarction, where it promotes apoptosis by acting as a sponge for miR‑29, thus revealing a noncoding RNA–mediated regulatory layer linked to the ACAP2 locus."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": " Finally, proteomic and structural analyses have identified ACAP2 as a bona fide effector of Rab35 that contributes to neurite outgrowth and further substantiates the critical interplay between the Rab35 and Arf6 signaling cascades."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Collectively, these studies highlight ACAP2 as a central regulator that links Rab35 and Arf6 signaling to control a spectrum of cellular processes—including endocytic recycling, actin dynamics, phagocytosis, and even virus–host interactions—while also revealing alternative regulatory functions via its circular RNA derivative.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "T R Jackson, F D Brown, Z Nie, et al. "}, {"type": "b", "children": [{"type": "t", "text": "ACAPs are arf6 GTPase-activating proteins that function in the cell periphery."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Biol (2000)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1083/jcb.151.3.627"}], "href": "https://doi.org/10.1083/jcb.151.3.627"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11062263"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11062263"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Youhei Egami, Mitsunori Fukuda, Nobukazu Araki "}, {"type": "b", "children": [{"type": "t", "text": "Rab35 regulates phagosome formation through recruitment of ACAP2 in macrophages during FcγR-mediated phagocytosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Sci (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/jcs.083881"}], "href": "https://doi.org/10.1242/jcs.083881"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22045739"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22045739"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Youhei Egami, Makoto Fujii, Katsuhisa Kawai, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Activation-Inactivation Cycling of Rab35 and ARF6 Is Required for Phagocytosis of Zymosan in RAW264 Macrophages."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Immunol Res (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1155/2015/429439"}], "href": "https://doi.org/10.1155/2015/429439"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26229970"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26229970"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Ritu R Bradley, Masanori Terajima "}, {"type": "b", "children": [{"type": "t", "text": "Vaccinia virus K1L protein mediates host-range function in RK-13 cells via ankyrin repeat and may interact with a cellular GTPase-activating protein."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Virus Res (2005)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.virusres.2005.06.003"}], "href": "https://doi.org/10.1016/j.virusres.2005.06.003"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16039000"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16039000"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Xiangzhi Meng, Yan Xiang "}, {"type": "b", "children": [{"type": "t", "text": "Vaccinia virus K1L protein supports viral replication in human and rabbit cells through a cell-type-specific set of its ankyrin repeat residues that are distinct from its binding site for ACAP2."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Virology (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.virol.2006.05.032"}], "href": "https://doi.org/10.1016/j.virol.2006.05.032"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16806385"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16806385"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Xue Liu, Meixiang Wang, Qiang Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CircRNA ACAP2 induces myocardial apoptosis after myocardial infarction by sponging miR-29."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Minerva Med (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.23736/S0026-4806.20.06600-8"}], "href": "https://doi.org/10.23736/S0026-4806.20.06600-8"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32406223"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32406223"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Eiko Kanno, Koutaro Ishibashi, Hotaka Kobayashi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Comprehensive screening for novel rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Traffic (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/j.1600-0854.2010.01038.x"}], "href": "https://doi.org/10.1111/j.1600-0854.2010.01038.x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20070612"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20070612"}]}]}]}
Synonyms	CNT-B2, CENTB2
Proteins	ACAP2_HUMAN
NCBI Gene ID	23527
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

ACAP2 has 6,006 functional associations with biological entities spanning 9 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA, sequence feature) extracted from 114 datasets.

Click the + buttons to view associations for ACAP2 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

ACAP2 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ACAP2 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of ACAP2 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of ACAP2 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of ACAP2 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ACAP2 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of ACAP2 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ACAP2 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ACAP2 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of ACAP2 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of ACAP2 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with ACAP2 protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with ACAP2 gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ACAP2 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of ACAP2 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of ACAP2 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of ACAP2 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing ACAP2 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing ACAP2 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing ACAP2 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing ACAP2 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with ACAP2 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with ACAP2 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of ACAP2 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with ACAP2 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with ACAP2 gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with ACAP2 gene/protein from the curated CTD Gene-Disease Associations dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by ACAP2 gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with ACAP2 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with ACAP2 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with ACAP2 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with ACAP2 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at ACAP2 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ACAP2 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of ACAP2 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing ACAP2 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of ACAP2 gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with ACAP2 gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing ACAP2 from the GeneSigDB Published Gene Signatures dataset.
	GEO Signatures of Differentially Expressed Genes for Diseases	disease perturbations changing expression of ACAP2 gene from the GEO Signatures of Differentially Expressed Genes for Diseases dataset.
	GEO Signatures of Differentially Expressed Genes for Gene Perturbations	gene perturbations changing expression of ACAP2 gene from the GEO Signatures of Differentially Expressed Genes for Gene Perturbations dataset.